Basically, the standard operating procedure outlined in this document consists of the following steps, namely, (i) preparation of the cells for the DCFH-DA assay, (ii) DCFH-DA test procedure, (iii) processing of the results. Practical details are provided for each of these steps and are followed by
Last updated on: 28-02-2022 - 10:59
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated
This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict otherwise
Last updated on: 28-02-2022 - 10:59
Contact: Vânia Vilas-Boas
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids (LipidTOX
Last updated on: 28-02-2022 - 10:58
This method assesses general cytotoxicity. Upon disruption of the cell membrane, lactate dehydrogenase (LDH) is released. LDH catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of reduced (NADH) and oxidized (NAD+) nicotinamide adenine dinucleotide. The principle
Last updated on: 28-02-2022 - 10:58
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to
Last updated on: 28-02-2022 - 10:58
Quantum Dots (QDs) have been hypothesized as potential probes for the optical monitoring of electrogenic cell activity given their high optical absorption cross-sections compared to organic fluorophores, high brightness and low photobleaching. Despite the theoretical predictions, less than a handful
Last updated on: 04-08-2021 - 16:50
Contact: Stijn Jooken
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have established organoid cultures from patient-derived ovarian cancer (OC), in particular from the most prevalent high-grade serous ovarian cancer (HGSOC). Testing multiple culture medium components identified neuregulin-1 (NRG1) as key factor in maximizing OC organoid development and growth,
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have developed multiple organoid models from a broad spectrum of endometrial pathologies that capture endometrial disease diversity and will provide powerful research models and drug screening and discovery tools. Organoids from endometriosis show disease-associated traits and cancer-linked
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have established organoids from mouse pituitary with the aim to generate a novel research model to study pituitary stem cell biology in both healthy and diseased glands. The organoids originated from the pituitary cells expressing the stem cell marker SOX2, were long-term expandable, displayed a
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We tissue-engineer in vitro, skeletal muscle consisting of aligned myofibers. To create the so-called bio-artificial muscle (BAM), human muscle progenitor cells are expanded, and a 3D construct is created by mixing the cells with a hydrogel. The cell-gel mix is cast into custom-made silicone molds
Last updated on: 08-12-2020 - 17:46
Contact: Lieven Thorrez
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
