The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a

Last updated on: 08-11-2019 - 09:17

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)

The RT-qPCR assay is used to identify genotoxic and non-genotoxic compounds. Herefore metabolic-competent human HepaRG cells are exposed to the IC10 value (measured by the MTT test). A microassay is performed to select 84 genes that show the most robust rates of correct classification of the

Last updated on: 08-11-2019 - 09:17

Organisation: Sciensano
Partners: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal

This method assesses general cytotoxicity. Upon disruption of the cell membrane, lactate dehydrogenase (LDH) is released. LDH catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of reduced (NADH) and oxidized (NAD+) nicotinamide adenine dinucleotide. The

Last updated on: 08-11-2019 - 09:17

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use

The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids

Last updated on: 08-11-2019 - 09:15

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use

The VITOTOX test is a high-throughput bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After

Last updated on: 08-11-2019 - 09:14

Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use

This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict

Last updated on: 08-11-2019 - 09:14

Organisation: Vrije Universiteit Brussel
Status: Still in development

The present standard procedure describes a protocol for measuring the urea concentration in supernatant of human stem cell-derived hepatocyte-like cells. This procedure relies on a chromogenic reagent that forms a colored complex specifically with urea. The latter can be measured and is directly

Last updated on: 08-11-2019 - 09:14

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated

Basically, the standard operating procedure outlined in this document consists of the following steps, namely, (i) preparation of the cells for the DCFH-DA assay, (ii) DCFH-DA test procedure, (iii) processing of the results. Practical details are provided for each of these steps and are followed

Last updated on: 08-11-2019 - 09:14

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated