Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells

Last updated on: 08-11-2019 - 09:19

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that are unable to migrate to the poles during the

Last updated on: 08-11-2019 - 09:18

Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)

The Ames test is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to permanent gene mutations. The test employs several histidine dependent Salmonella strains each carrying different

Last updated on: 08-11-2019 - 09:18

Contact: Roel Anthonissen
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)

The Alkaline Comet Assay is a microgel electrophoresis technique which allows to measure DNA damage (single and double strand breaks, alkali labile sites, incomplete excision repair sites and cross links) cell by cell. Cells are mixed with 0.8% Low Melting Point Agarose which is spread as a gel

Last updated on: 08-11-2019 - 09:18

Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use

The neutral red uptake (NRU) assay provides a quantitative measurement of the number of viable cells. The test is based on the ability of living cells to take up and bind neutral red (NR), a dye which easily penetrates cell membranes via non-ionic diffusion. It accumulates in the lysosomes. Dying

Last updated on: 08-11-2019 - 09:18

Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use

Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the

Last updated on: 08-11-2019 - 09:17

Contact: Matthias Rombaut
Organisation: Vrije Universiteit Brussel
Status: History of use

The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a

Last updated on: 08-11-2019 - 09:17

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)

The RT-qPCR assay is used to identify genotoxic and non-genotoxic compounds. Herefore metabolic-competent human HepaRG cells are exposed to the IC10 value (measured by the MTT test). A microassay is performed to select 84 genes that show the most robust rates of correct classification of the

Last updated on: 08-11-2019 - 09:17

Organisation: Sciensano
Partners: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal

This method assesses general cytotoxicity. Upon disruption of the cell membrane, lactate dehydrogenase (LDH) is released. LDH catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of reduced (NADH) and oxidized (NAD+) nicotinamide adenine dinucleotide. The

Last updated on: 08-11-2019 - 09:17

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use