HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The

Last updated on: 08-11-2019 - 10:11

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel
Status: History of use

Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) express several key liver-specific transcription factors as well as hepatic progenitor markers. However, they still lack the hepatocyte nuclear factors 1-alpha (HNF1a) and 4-alpha (HNF4a), indispensable for their reprogramming

Last updated on: 08-11-2019 - 10:09

Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel
Status: Still in development

Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells.

Last updated on: 08-11-2019 - 09:52

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use

Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine

Last updated on: 08-11-2019 - 09:49

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular

Last updated on: 08-11-2019 - 09:48

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

This method provides a new and easy to standardize enzymatic isolation protocol to obtain human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). hUC-MSCs are obtained within 3 hours and the isolation method provides a minimal risk of bacterial contamination. The so-obtained hUC-MSCs

Last updated on: 08-11-2019 - 09:46

Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

Cultivated liver cells are fixed with 4% (w/v) paraformaldehyde (PFA) for 10 minutes at room temperature and subsequently incubated for 15 minutes with 100 mM glycin solution, used to saturate reactive groups generated after PFA fixation. These cells are subsequently incubated for 10 minutes with

Last updated on: 08-11-2019 - 09:44

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal

Rat liver epithelial cells are cultivated at 100% confluency on 100 µg/mL rat tail collagen type I coated culture dishes in base medium and sequentially exposed to hepatogenic growth factors and cytokines. Base medium consisted of William’s E medium without glutamine supplemented with 7.33 IE/mL

Last updated on: 08-11-2019 - 09:43

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal

Cryopreserved differentiated HepaRG cells (obtained from Biopredic International) are derived from a human hepatocellular carcinoma. These cells are an unique in vitro tool that provides reproducible results and exhibit many characteristics of primary human hepatocytes such as morphology

Last updated on: 08-11-2019 - 09:42

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel
Status: History of use