We describe a fluorescent cell-based in vitro infection model that reproduces host-Batrachochytrium dendrobatidis (Bd) interactions. Using the epithelial cell line A6 from Xenopus laevis, we reproduced different stages of host cell infection and intracellular growth of Bd, resulting in host cell

Last updated on: 10-01-2020 - 15:28

Contact: Elin Verbrugghe
Organisation: Ghent University
Status: Published in peer reviewed journal
We have successfully set-up the iPSC technology and are able to derive human cortical neurons for the study of neurodevelopmental disorders e.g. the MECP2 duplication syndrome (published) and other projects in the lab (ongoing). We also create isogenic lines using CrispR-Cas technology. All lines

Last updated on: 07-01-2020 - 15:23

Contact: Hilde Van Esch
Organisation: KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal
Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro experiments would be

Last updated on: 06-01-2020 - 17:04

Contact: Daniele Parisi
Organisation: KU Leuven
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development
In order to find a solution for the center-weighted opacity reading associated with the OP-KIT opacitometer, a prototype of a laser light-based opacitometer (PLLBO) allowing better measurement of opacities was developed (Van Goethem et al., 2010; Annex 1). The technical optimization and optical

Last updated on: 06-01-2020 - 16:59

Contact: An Van Rompay
Organisation: VITO
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. The protocol deals with exposing zebrafish embryos to a range of compound concentrations at 28°C

Last updated on: 06-01-2020 - 16:55

Organisation: University of Antwerp
Status: Still in development, Published in peer reviewed journal
The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the endothelium.

Last updated on: 03-12-2019 - 17:23

Organisation: UCLouvain
Partners: UCLouvain
Status: Internally validated
The lab routinely use C. elegans culture as genetic model. We explore the development and the ageing of the neuromuscular system. The methods used regularly in our lab include molecular biology, generation of transgenic animals or mutants, crossings, genetic screen, calcium imaging, fluorescence

Last updated on: 02-12-2019 - 13:54

Contact: patrick laurent
Organisation: Université libre de Bruxelles
Status: Internally validated, Published in peer reviewed journal
The cytokinesis-block micronucleus method allows assessing the presence of DNA damage at the chromosome level. It is an essential part of toxicology, because mutation is a crucial event in carcinogenesis. The capacity of inhaled particles to induce irreversible mutations in type II lung epithelial

Last updated on: 26-11-2019 - 17:35

Organisation: UCLouvain
Status: Validated by an external party (e.g. OECD, EURL ECVAM,…)
Cell Culture and Reagents hiPSC-CMs can be obtained commercially either as living pre-plated cells seeded onto fibronectin-coated 96-well mClear plates (Greiner Bio-One, No. 655090) or can be plated in house at a density (~25,000 cells/well) suited to forming a confluent synchronously beating mono

Last updated on: 26-11-2019 - 13:17

Contact: Ard Teisman
Organisation: Janssen Pharma of JNJ
Status: Internally validated, Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured

Last updated on: 26-11-2019 - 13:12

Contact: Kristel Demeyere
Organisation: Ghent University
Status: Published in peer reviewed journal