For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the

Last updated on: 22-03-2019 - 11:36

Organisation: Vrije Universiteit Brussel
Status: Still in development

Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs [1]. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem

Last updated on: 22-03-2019 - 11:36

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use

ATP transports chemical energy within cells by serving as a substrate for kinases and as such fulfills a vital function in numerous cellular processes such as cell injury and subsequent cell death. ATP is therefore a crucial player in these events that are results of intracellular stress.

Last updated on: 22-03-2019 - 11:35

Contact: Axelle Cooreman
Organisation: Vrije Universiteit Brussel
Status: History of use

The MTT test is performed to determine the in vitro cytotoxicity of selected chemicals. The mitochondrial enzyme succinate deydrogenase is responsible for the biotransformation of toxic agents and MTT. The ability of cells to reduce MTT provides an indication of the mitochondrial integrity and

Last updated on: 22-03-2019 - 11:35

Contact: Axelle Cooreman
Organisation: Vrije Universiteit Brussel
Status: History of use

The present standard procedure describes a protocol for measuring the urea concentration in supernatant of human stem cell-derived hepatocyte-like cells. This procedure relies on a chromogenic reagent that forms a colored complex specifically with urea. The latter can be measured and is directly

Last updated on: 22-03-2019 - 11:30

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated

Basically, the standard operating procedure outlined in this document consists of the following steps, namely, (i) preparation of the cells for the DCFH-DA assay, (ii) DCFH-DA test procedure, (v) processing of the results. Practical details are provided for each of these steps and are followed by

Last updated on: 22-03-2019 - 11:30

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated

This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict otherwise

Last updated on: 22-03-2019 - 11:29

Organisation: Vrije Universiteit Brussel
Status: Still in development

The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids

Last updated on: 22-03-2019 - 11:29

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use

This method assesses general cytotoxicity. Upon disruption of the cell membrane, lactate dehydrogenase (LDH) is released. LDH catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of reduced (NADH) and oxidized (NAD+) nicotinamide adenine dinucleotide. The

Last updated on: 22-03-2019 - 11:28

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel
Status: History of use

Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line

Last updated on: 22-03-2019 - 11:28

Contact: Matthias Rombaut
Organisation: Vrije Universiteit Brussel
Status: History of use