This method provides a new and easy to standardize enzymatic isolation protocol to obtain human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). hUC-MSCs are obtained within 3 hours and the isolation method provides a minimal risk of bacterial contamination. The so-obtained hUC-MSCs were

Last updated on: 08-11-2019 - 09:46

Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal
By the use of monolayer cultures as an in vitro system, the effects of drugs on CYP3A activity can be evaluated. It relies on the use of a luminogenic CYP3A substrate, namely luciferin-6′-pentafluorobenzylether (luciferin- PFBE). Upon biotransformation by CYP3A, luciferin-PFBE is converted into

Last updated on: 08-11-2019 - 09:45

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use
Cultivated liver cells are fixed with 4% (w/v) paraformaldehyde (PFA) for 10 minutes at room temperature and subsequently incubated for 15 minutes with 100 mM glycin solution, used to saturate reactive groups generated after PFA fixation. These cells are subsequently incubated for 10 minutes with 1%

Last updated on: 08-11-2019 - 09:44

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Rat liver epithelial cells are cultivated at 100% confluency on 100 µg/mL rat tail collagen type I coated culture dishes in base medium and sequentially exposed to hepatogenic growth factors and cytokines. Base medium consisted of William’s E medium without glutamine supplemented with 7.33 IE/mL

Last updated on: 08-11-2019 - 09:43

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Cryopreserved differentiated HepaRG cells (obtained from Biopredic International) are derived from a human hepatocellular carcinoma. These cells are an unique in vitro tool that provides reproducible results and exhibit many characteristics of primary human hepatocytes such as morphology, expression

Last updated on: 08-11-2019 - 09:42

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel
Status: History of use
Rat liver epithelial cells (rLEC) can be isolated from 8-day old male Sprague-Dawley rats. Briefly, small fragments of neonatal rat livers are incubated for 15 minutes with 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffered trypsin solution [0.25% (v/v)] and washed twice with

Last updated on: 08-11-2019 - 09:40

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417

Last updated on: 08-11-2019 - 09:39

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Internally validated
Mononuclear cells (MNC) are isolated from bone marrow aspirates by density gradient centrifugation and washed in Hank's buffered salt solution. MNC are seeded at a cell density of 2 × 10E4 cells/cm2 in low glucose DMEM supplemented with 15% (v/v) heat-inactivated FBS, 2 mM L-glutamine and 0.5% (v/v)

Last updated on: 08-11-2019 - 09:33

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Approximately 125 g of processed adipose tissue is incubated for 90 minutes at 37°C in dissociation medium (1:1) consisting of 1% (v/v) bovine serum albumin and 1 mg/mL collagenase A in phosphate buffered saline (PBS). After two filtration steps, the filtrate is carefully brought on top of 15 mL of

Last updated on: 08-11-2019 - 09:32

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Human skin-derived precursor cells (hSKP) between are cultivated at 90% confluency on 100 µg/mL collagen type I in base medium and sequentially exposed to hepatogenic factors. Base medium consists of Dulbecco’s Modified Eagle Medium + GLUTAMAX / F12 Nutrient supplement (3:1), supplemented with 7.33

Last updated on: 08-11-2019 - 09:31

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal