Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin

Last updated on: 08-11-2019 - 09:13

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel
Status: History of use

The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a

Last updated on: 08-11-2019 - 09:07

Organisation: Vrije Universiteit Brussel
Status: Still in development

Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation

Last updated on: 08-11-2019 - 09:04

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix

Last updated on: 08-11-2019 - 09:03

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development

Loss of acinar differentiation drives pancreatic cancer. An established human in vitro experimental model is used in our lab to study this process. Pancreatic exocrine cells from human donors are placed in suspension culture in Advanced RPMI medium supplemented with 5% heat-inactivated fetal

Last updated on: 21-08-2019 - 11:36

Contact: Elyne Backx
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal