3D organoid culture of MMTV-PyMT mammary gland tumors

The mammary-specific polyomavirus middle T antigen overexpression mouse model (MMTV-PyMT) is one of the most commonly used models in the cancer research field for multiple reasons, among which the spontaneous development of multifocal luminal tumors, the early tumoral onset, and the primary tumors'

Last updated on: 11-10-2024 - 15:19

Contact: Colinda Scheele
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
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A mouse mammary gland organoid protocol to mimic breast morphology in vitro

The protocol is aimed at developing primary mammary gland organoids that have a morphology similar to the one of the in vivo breast, which is organized as a complex network of interconnected branches. The organoids are derived from the mouse mammary gland by mechanical dissociation and enzymatic

Last updated on: 11-10-2024 - 15:19

Contact: Colinda Scheele
Organisation: Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
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Soft Matrix for Tissue engineeRing and in vitro modelling

The extracellular matrix (ECM) is a highly organized tissue-specific network, which is secreted by tissue-resident cells and significantly influences cell phenotype, behaviour and function. The 3R principle calls for the replacement of animal models in areas like drug screening, and consequently,

Last updated on: 27-09-2024 - 16:39

Contact: Marguerite Meeremans
Organisation: Ghent University (UGent)
Status: Still in development
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How insulin shapes lung development in a new human stem cell organoid model

Respiratory diseases are one of the leading causes of mortality. Despite the efforts to understand lung development, physiology, and pathology, this field remains in its early stages. In vitro models can replicate lung physiology to comprehend development, function, and pathology. Lung organoids

Last updated on: 25-09-2024 - 11:44

Contact: Alessandra Boggian
Organisation: Université Libre de Bruxelles (ULB)
Partners: Université Libre de Bruxelles (ULB)
Status: Still in development
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Human liver spheroid co-cultures to investigate parenteral nutrition-induced hepatotoxicity

Total Parenteral Nutrition (TPN) can cause adverse effects, including metabolic disorders and liver injury. TPN-associated liver injury, known as intestinal failure-associated liver disease (IFALD), represents a significant problem affecting up to 90% of individuals receiving TPN. Despite numerous

Last updated on: 10-09-2024 - 13:54

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
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Human Dental Pulp Stem Cells as a Patient-in-a-Dish Model for Charcot-Marie-Tooth Disease Type 1A

Dental pulp stem cells (DPSC) are mesenchymal stem cells residing within the inner mucoid core (dental pulp) of teeth, responsible for tissue turnover and regeneration. Since third molars, or wisdom teeth, are frequently extracted for orthodontic reasons, DPSC are a highly accessible stem cell

Last updated on: 23-08-2024 - 11:01

Contact: Nathalie Dirkx
Organisation: University of Hasselt (UHasselt)
Partners: University of Hasselt (UHasselt)
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal
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(Equine) in vitro Tendinopathy Model

Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes. Despite the big burden, there is no effective treatment to restore tendon’s natural composition, due to a lack of understanding of fundamental cell biology. Additionally, translation of novel

Last updated on: 12-08-2024 - 09:32

Contact: Marguerite Meeremans
Organisation: Ghent University (UGent)
Status: Still in development
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Organoids of synovial sarcoma

The use of three-dimensional (3D) cell culture models is considered a reliable model that is successfully used for high-throughput drug testing in carcinoma research. However, the development of soft tissue sarcoma organoids is lagging behind. Soft tissue sarcoma is a group of rare malignancies from

Last updated on: 30-07-2024 - 12:08

Contact: Agnieszka Wozniak
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Still in development
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Colon-on-a-plate

The Colon-on-a-plate® technology is a high-throughput biorelevant in vitro simulation of the physiology and microbiology of the colon. This robust screening technology is not limited to comparing the impact of tens of test product on the microbiome, but also offers insight into the factors

Last updated on: 26-07-2024 - 09:50

Contact: Cannot be disclosed
Organisation: ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
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Simulator of Human Intestinal Microbial Ecosystem

The SHIME® (Simulator of Human Intestinal Microbial Ecosystem) model is currently the most representative in vitro technology for the combined simulation of the physiological, chemical and microbiological properties of the gastrointestinal tract. The model enables study of the impact of long-term

Last updated on: 26-07-2024 - 09:49

Contact: Cannot be disclosed
Organisation: ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
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