Using the combination of advanced CRIPSR tools including several Cas orthologs, based editors (ABE, CBE, CGBE) and prime editing technologie with efficient delivery vehicles such as LV, AAV and virus-like particles (VLPs), our lab has become experienced with introducing or replacing precise edits in

Last updated on: 21-06-2024 - 11:29

Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated
The DETECTOR algorithm allows to functionally screen genetic strategies for Cystic Fibrosis in patient-derived rectal organoids. DETECTOR is a machine-learning based software that takes frames from forskolin-induced swelling (FIS) assay on organoids as input and gives the number of functionally

Last updated on: 10-06-2024 - 11:17

Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are

Last updated on: 22-02-2024 - 11:15

Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem cells

Last updated on: 20-02-2024 - 11:27

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Adverse outcome pathway (AOP) networks are versatile tools in toxicology and risk assessment that capture and visualize mechanisms driving toxicity originating from various data sources. They share a common structure consisting of a set of molecular initiating events and key events, connected by key

Last updated on: 20-02-2024 - 09:27

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
Drug-induced intrahepatic cholestasis (DIC) is a main type of hepatic toxicity that is challenging to predict in early drug development stages. Preclinical animal studies often fail to detect DIC in humans. In vitro toxicogenomics assays using human liver cells have become a practical approach to

Last updated on: 15-02-2024 - 11:59

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
Alkaptonuria (AKU) is a rare inborn error of metabolism caused by a defective homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the tyrosine degradation pathway. Loss of HGD function leads to the accumulation of homogentisic acid (HGA) in connective body tissues in a process called

Last updated on: 06-12-2023 - 14:45

Contact: Sien Lequeue
Organisation: Vrije Universiteit Brussel (VUB)
Partners: RWTH Aachen
Status: Published in peer reviewed journal
Zebrafish-based platforms have recently emerged as a useful tool for toxicity testing as they combine the advantages of in vitro and in vivo methodologies. Nevertheless, the capacity to metabolically convert xenobiotics by zebrafish eleuthero embryos is supposedly low. To circumvent this concern, a

Last updated on: 01-09-2023 - 09:55

Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The spatiotemporal distribution of fluorescent compounds was examined during 48 h after immersion (10 µM) or microinjection (2 mg/kg) in the pericardial cavity (PC), intraperitoneally (IP) and yolk sac (IY) of 3 dpf zebrafsh eleuthero-embryos. By modelling the fluorescence of whole-body contours

Last updated on: 01-09-2023 - 09:43

Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Immersion treatment: zebrafish embryos at the two-cell stage were immersed in Danieau’s medium containing the compound. A compound concentration of 10 µM and DMSO concentration of 0.1% (v/v) in a volume of 5 mL was used per well (6-well-plates). At 72 h, the zebrafish medium containing the compound

Last updated on: 01-09-2023 - 09:32

Contact: Marie Pardon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal