The tetanus neurotoxin (TeNT) is one of the most toxic proteins known to man, which prior to the use of the vaccine against the TeNT producing bacteria Clostridium tetani, resulted in a 20% mortality rate upon infection. The clinical detrimental effects of tetanus have decreased immensely since the
Last updated on: 22-03-2022 - 16:49
Lung cancer thrives in a complex multicellular tumor microenvironment (TME) that impacts tumor growth, metastasis, response, and resistance to therapy. While orthotopic murine lung cancer models can partly recapitulate this complexity, they do not resonate with high-throughput immunotherapeutic drug
Last updated on: 16-03-2022 - 14:53
Contact: Cleo Goyvaerts
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Institut Curie
Status: Published in peer reviewed journal
This model is a 3D human co-culture model where both hepatocyte functionality and HSC quiescence can be maintained for at least 21 days. This novel system allows hepatotoxicity testing and can detect drug-induced as well as hepatocyte-dependent HSC activation, thereby representing an important step
Last updated on: 16-03-2022 - 14:51
Generation of Hepatic Stellate Cells from Human Pluripotent Stem for in vitro liver fibrosis studies
We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance
Last updated on: 16-03-2022 - 14:49
Contact: Leo A. van Grunsven
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and
Last updated on: 16-03-2022 - 14:47
Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes
Last updated on: 16-03-2022 - 14:32
Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and
Last updated on: 16-03-2022 - 14:23
Contact: Wendy Meulebroeck
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
We developed a high-throughput live-cell imaging-based organoid analysis platform, Organoid Brightfield Identification-based Therapy Screening (Orbits), that allows for kinetic monitoring of organoid growth and drug responses in routine extracellular matrix domes, high-throughput 384-well
Last updated on: 16-03-2022 - 13:59
Contact: Christophe Deben
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human cells
Last updated on: 28-02-2022 - 11:01
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use
Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417
Last updated on: 28-02-2022 - 11:00
Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated
