Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are
Last updated on: 22-02-2024 - 11:15
Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
UHDR irradiations show healthy tissue sparing effect known as the FLASH effect. Since 2014, the FLASH effect is investigated worldwide to understand how it works and how to trigger it. The FLASH effect is defined as an in vivo effect. However, in vivo models are often expensive and time-consuming.
Last updated on: 05-09-2023 - 08:45
Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of
Last updated on: 11-01-2023 - 16:43
Contact: Bram Verstockt
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are
Last updated on: 13-12-2022 - 11:23
Contact: Frédéric Clotman
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a
Last updated on: 26-05-2022 - 10:39
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and
Last updated on: 16-03-2022 - 14:47
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,
Last updated on: 16-03-2022 - 14:45
Contact: Charles Van der Henst
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration
Last updated on: 28-02-2022 - 11:04
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The cardiorespiratory model reproduces human physiology with a high level of fidelity. It is a lumped parameter model including a representation of atria, ventricles, pulmonary and systemic circulations, autonomic controls, metabolic peripheral control, ventilation and gas exchange in tissues and
Last updated on: 21-10-2021 - 10:07
Contact: Libera Fresiello
Organisation: Cannot be disclosed
Partners: Cannot be disclosed
Status: History of use, Internally validated, Published in peer reviewed journal
We aimed to optimize fibrin matrix composition in order to mimic human ovarian tissue architecture for human ovarian follicle encapsulation and grafting. Ultrastructure of fresh human ovarian cortex in age-related women (n = 3) and different fibrin formulations (F12.5/T1, F30/T50, F50/T50, F75/T75),
Last updated on: 10-09-2021 - 14:18
Contact: Christiani Amorim
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
