Using the combination of advanced CRIPSR tools including several Cas orthologs, based editors (ABE, CBE, CGBE) and prime editing technologie with efficient delivery vehicles such as LV, AAV and virus-like particles (VLPs), our lab has become experienced with introducing or replacing precise edits in
Last updated on: 21-06-2024 - 11:29
Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated
The DETECTOR algorithm allows to functionally screen genetic strategies for Cystic Fibrosis in patient-derived rectal organoids. DETECTOR is a machine-learning based software that takes frames from forskolin-induced swelling (FIS) assay on organoids as input and gives the number of functionally
Last updated on: 10-06-2024 - 11:17
Contact: Marianne Carlon
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are
Last updated on: 22-02-2024 - 11:15
Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Drug-induced phospholipidosis (DIPL) is a metabolic disorder characterized by an excessive intracellular accumulation of phospholipids caused by cationic drugs. Hepatic cells derived from human skin are evaluated as an in vitro model to investigate DIPL and its mechanisms. Human skin stem cells
Last updated on: 20-02-2024 - 11:27
Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
The culture of primary human synoviocytes provides an excellent cellular model for studying the normal and pathological physiology of synoviocytes and the development of joint diseases. Human primary synoviocytes can either be provided by commercial suppliers or isolated from fresh biological
Last updated on: 17-01-2024 - 11:32
Alkaptonuria (AKU) is a rare inborn error of metabolism caused by a defective homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the tyrosine degradation pathway. Loss of HGD function leads to the accumulation of homogentisic acid (HGA) in connective body tissues in a process called
Last updated on: 06-12-2023 - 14:45
Contact: Sien Lequeue
Organisation: Vrije Universiteit Brussel (VUB)
Partners: RWTH Aachen
Status: Published in peer reviewed journal
More than 3 million deaths worldwide are linked to excessive alcohol consumption. Alcohol abuse associated gut barrier dysfunction is thought to play an important role in the development of alcohol-associated liver disease. Although some aspects that contribute to this process have been elucidated,
Last updated on: 30-10-2023 - 10:05
Contact: Ami Gloria Toulehohoun
Organisation: Université Catholique de Louvain (UCL)
Partners: UCLouvain, Cliniques universitaires Saint-Luc, UCLouvain
Status: Still in development
The maturation of the human brain shows species-specific differences of neoteny when compared to lower mammals. This process encompass a time window that expands from late embryonic stages to early adolescence. Major features of brain maturation are the acquisition of phenotypic complex traits such
Last updated on: 25-09-2023 - 14:38
Contact: Ira Espuny Camacho
Organisation: University of Liège (ULiège)
Status: Still in development, History of use
The use of patient-derived primary cell cultures in cancer preclinical assays, including drug screens and genotoxic studies, has increased in recent years. However, their translational value is constrained by several limitations, including variability that can be caused by the culture conditions.
Last updated on: 20-07-2023 - 15:05
The Systemic Intestinal Fermentation Research technology, SIFR in short, is a uniquely validated simulation of gut microbial ecosystems, shown to be predictive for clinical outcomes. The SIFR can simulate a wide variety of gut microbiota from humans (infants, adults, elderly; healthy, diseased) to
Last updated on: 08-06-2023 - 14:24
Contact: Aurélien Baudot
Organisation: Cryptobiotix
Status: History of use, Internally validated, Published in peer reviewed journal
