The use of patient-derived primary cell cultures in cancer preclinical assays, including drug screens and genotoxic studies, has increased in recent years. However, their translational value is constrained by several limitations, including variability that can be caused by the culture conditions.
Last updated on: 20-07-2023 - 15:05
The Systemic Intestinal Fermentation Research technology, SIFR in short, is a uniquely validated simulation of gut microbial ecosystems, shown to be predictive for clinical outcomes. The SIFR can simulate a wide variety of gut microbiota from humans (infants, adults, elderly; healthy, diseased) to
Last updated on: 08-06-2023 - 14:24
Contact: Aurélien Baudot
Organisation: Cryptobiotix
Status: History of use, Internally validated, Published in peer reviewed journal
Using an in vitro flow system adhesion assay, immune cell adhesion to (blood-brain barrier) endothelial cells can be quantified under physiological blood flow conditions. Endothelial cells can be treated with inflammatory cytokines or therapeutic antibodies to mimic inflammatory diseases. Immune
Last updated on: 06-06-2023 - 10:35
Contact: Doryssa Hermans
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
ProDigest's short-term colonic simulation is an in vitro model for rapid screening of the interaction between test products and the gut microbiome. The key microbial saccharolytic and proteolytic markers are analysed as well as evolution in the composition of the microbiome and other endpoints as
Last updated on: 17-04-2023 - 13:22
Contact: Cannot be disclosed
Organisation: ProDigest
Partners: ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
Macrophages orchestrate reactive particle segregation, compact aggregates of immune cells and non-immune cells and promote fibrosis-surrounding granulomas.
We developed a simple 3D in-vitro model that mimics granuloma formation and categorizes granuloma-inducing inorganic particles. Macrophage
Last updated on: 03-04-2023 - 11:21
Contact: Léa Hiéronimus
Organisation: Université Catholique de Louvain (UCL)
Status: Still in development, Internally validated
We aim to differentiate ovarian cells isolated from postmenopausal ovaries into steroidogenic theca interna cells. For this purpose, cryopreserved ovarian cortex fragments are used to isolate ovarian cells. These cells are cultured on collagen and supplemented with growth factors defined to promote
Last updated on: 20-03-2023 - 13:52
Contact: Hanne Vlieghe
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
Generally, our research group aims to study the influence of food parameters (food design, food processing, food composition) on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use in vitro digestion protocols. The current method is a semi
Last updated on: 15-02-2023 - 16:08
Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Primary epithelial stem/progenitor cell populations are isolated from the murine lung using fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS). Purified epithelial stem/progenitor cells are grown together with mesenchymal cells in Matrigel, a material enriched for
Last updated on: 15-02-2023 - 10:50
Contact: Smole Ursula
Organisation: VIB - UGent
Status: Still in development, Published in peer reviewed journal
This method is used to let cells interact for a better simulation of processes occurring in the body. The rational of the method is that monoculture experiments do not capture the complexity of in vivo interactions between different organs. In the most simple setup (published work Nutrients), Caco-2
Last updated on: 09-02-2023 - 16:37
Contact: Amar van Laar
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and
Last updated on: 31-01-2023 - 15:52
