Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes
Last updated on: 16-03-2022 - 14:32
Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.
Last updated on: 16-03-2022 - 14:28
Contact: Surajit Sahu
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Published in peer reviewed journal
Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.
Last updated on: 16-03-2022 - 14:25
Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and
Last updated on: 16-03-2022 - 14:23
Contact: Wendy Meulebroeck
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Loss of acinar differentiation drives pancreatic cancer. An established human in vitro experimental model is used in our lab to study this process. Pancreatic exocrine cells from human donors are placed in suspension culture in Advanced RPMI medium supplemented with 5% heat-inactivated fetal bovine
Last updated on: 16-03-2022 - 14:19
Contact: Elyne Backx
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration
Last updated on: 28-02-2022 - 11:04
Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells. Standards
Last updated on: 28-02-2022 - 11:02
This method provides a new and easy to standardize enzymatic isolation protocol to obtain human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). hUC-MSCs are obtained within 3 hours and the isolation method provides a minimal risk of bacterial contamination. The so-obtained hUC-MSCs were
Last updated on: 28-02-2022 - 11:02
Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) express several key liver-specific transcription factors as well as hepatic progenitor markers. However, they still lack the hepatocyte nuclear factors 1-alpha (HNF1a) and 4-alpha (HNF4a), indispensable for their reprogramming towards
Last updated on: 28-02-2022 - 11:02
HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The cells secrete
Last updated on: 28-02-2022 - 11:02
