We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the

Last updated on: 04-02-2020 - 13:31

Organisation: Ghent University, Ghent University, Liège Université
Status: Internally validated

The GIDM-colon is a validated in vitro model that allows the study of the availability of compounds and the metabolisation at the level of the colon. The human physiological conditions of the gastrointestinal tract (stomach, small intestine and colon) are mimicked. The continiuous flow

Last updated on: 03-02-2020 - 08:44

Organisation: University of Antwerp
Status: History of use, Internally validated, Published in peer reviewed journal

MELN cells [provided by INSERM, Montpellier, FR; Balaguer et al. (1999)] are oestrogen-sensitive human breast cancer cells (MCF-7) stably transfected with the oestrogen-responsive gene (ERE-βGlo-Luc-SVNeo) carried by integrated plasmids. A standard set-up has been developed to

Last updated on: 28-01-2020 - 16:39

Contact: Cannot be disclosed
Organisation: VITO
Status: History of use, Internally validated, Published in peer reviewed journal

The rainbow trout gill cell line assay quantifies cell viability using fluorescent measurements for metabolic activity (Alamar Blue, AB), cell membrane integrity (5-CarboxyFluorescein DiAcetate AcetoxyMethyl ester, CFDA-AM) and lysosomal membrane integrity (Neutral Red, NR). Chemicals are added

Last updated on: 20-01-2020 - 10:26

Contact: Cannot be disclosed
Organisation: VITO
Status: History of use, Internally validated, Published in peer reviewed journal

We have successfully set-up the iPSC technology and are able to derive human cortical neurons for the study of neurodevelopmental disorders e.g. the MECP2 duplication syndrome (published) and other projects in the lab (ongoing). We also create isogenic lines using CrispR-Cas technology. All lines

Last updated on: 07-01-2020 - 15:23

Contact: Hilde Van Esch
Organisation: KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal

Identifying drug-target interactions is a crucial step in drug repositioning, the process of suggesting new indications for known drugs. There are about 9000 FDA-approved and experimental small molecule drugs and more than 500.000 protein records available. Performing in vitro

Last updated on: 06-01-2020 - 17:04

Contact: Daniele Parisi
Organisation: KU Leuven
Partners: Biotech/TU-Dresden, Max-Planck-Institut für Informatik Saarbrucken
Status: Still in development

In order to find a solution for the center-weighted opacity reading associated with the OP-KIT opacitometer, a prototype of a laser light-based opacitometer (PLLBO) allowing better measurement of opacities was developed (Van Goethem et al., 2010; Annex 1). The technical optimization and optical

Last updated on: 06-01-2020 - 16:59

Contact: An Van Rompay
Organisation: VITO
Status: Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)

In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. The protocol deals with exposing zebrafish embryos to a range of compound

Last updated on: 06-01-2020 - 16:55

Organisation: University of Antwerp
Status: Still in development, Published in peer reviewed journal

The ex-vivo endothelium-stent contact model allows measuring the impact of implants on the arterial tissue. The endothelium is directly exposed by inserting a wire made of the investigated material into the lumen of the aortic ring to mimic the contact between the coronary stent and the

Last updated on: 03-12-2019 - 17:23

Organisation: UCLouvain
Partners: UCLouvain
Status: Internally validated