The application of liver organoids is very promising in the field of liver tissue engineering; however, it is still facing some limitations. One of the current major limitations is the matrix in which they are cultured. The mainly undefined and murine-originated tumor matrices derived from
Last updated on: 22-01-2024 - 17:28
Contact: Nathan Carpentier
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Computational neuroscience aims to study the nervous system by mathematical and computer simulations. Computational models can be built on multilevel scales. With the bottom-up approach, the model is built from the same building blocks as observed in human or animal tissue. As such, the functioning
Last updated on: 13-02-2023 - 16:45
Contact: Cannot be disclosed
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
This method is used to let cells interact for a better simulation of processes occurring in the body. The rational of the method is that monoculture experiments do not capture the complexity of in vivo interactions between different organs. In the most simple setup (published work Nutrients), Caco-2
Last updated on: 09-02-2023 - 16:37
Contact: Amar van Laar
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes. Despite the big burden, there is no effective treatment to restore tendon’s natural composition, due to a lack of understanding of fundamental cell biology. Additionally, translation of novel
Last updated on: 17-01-2023 - 16:22
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that
Last updated on: 23-11-2022 - 16:31
Contact: Birgit Mertens
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic
Last updated on: 14-10-2022 - 11:37
Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The VITOTOX test is a bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controlled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After incubation
Last updated on: 26-05-2022 - 10:40
Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The CALUX® system (Chemically Actived LUciferase eXpression) of Bio Detection Systems (BDS, Amsterdam, the Netherlands) uses U-2 OS cells (human osteoblast) that are stably transfected with human TRb or human PPARg2 (BDS, Amsterdam) and a luciferase reporter construct under the control of a receptor
Last updated on: 05-05-2022 - 08:59
Contact: Cannot be disclosed
Organisation: Sciensano
Partners: BDS
Status: Published in peer reviewed journal
This method provides a rapid untargeted screening metabolomics method, which can be used to evaluate whether or not metabolic changes can be appointed according to the metabolic state in your matrix of choice. It applies an ambient ionization technique in which a laser beam or diathermy probe is
Last updated on: 31-03-2022 - 15:31
Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
OECD TG 249 was developed by Prof. Dr. Kristin Schirmer, from Eawag. The OECD 249 aims at assessing the acute toxicity of single or mixtures of chemicals using the permanent rainbow trout gill cell line, RTgill-W1. RTgill-W1 cells in a confluent monolayer in 24-well plates are exposed to the test
Last updated on: 30-03-2022 - 17:15
Contact: João Barbosa
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
