The response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol was investigated using primary sublingual epithelial cells isolated from dogs and cultured in vitro. After 24-h incubation with

Last updated on: 07-04-2021 - 14:52

Contact: Eric Cox
Organisation: Ghent University
Status: Published in peer reviewed journal
This is a mathematical compartmental formulation of dose-effect synergy modelling for multiple therapies in Non Small Cell Lung Cancer (NSCLC): antiangiogenic, immuno- and radiotherapy. The model formulates the dose-effect relationship in a unified context, with tumor proliferating rates and

Last updated on: 01-02-2021 - 14:32

Organisation: Ghent University
Status: Published in peer reviewed journal
Computational Fluid Dynamics (CFD) is being applied to characterize the fluid flow in different applications. CFD has obtained significant interest in both the medical and engineering community because of its non-invasive character. It can predict the fluid flow characteristics when one or multiple

Last updated on: 10-04-2020 - 09:22

Organisation: Ghent University
Status: Still in development, Published in peer reviewed journal
This is a method to study host-microbe interaction in the upper respiratory tract. A physiologically representative epithelial structure, with mucin producing and ciliated cells, is obtained by culturing respiratory epithelial cells at air-liquid interface in Transwell inserts. Optionally,

Last updated on: 28-02-2020 - 10:51

Organisation: Ghent University
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal
It is the goal of the DNA-adductomics to search for DNA-adducts that might be formed during interaction with contaminants. The analysis of DNA adducts is performed using ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the

Last updated on: 12-11-2019 - 11:16

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: Internally validated, Published in peer reviewed journal
Our polar metabolomics profiling and fingerprinting methodology applies ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocols for colon tissue, cell cultures, urine,

Last updated on: 12-11-2019 - 11:13

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as

Last updated on: 12-11-2019 - 10:56

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: Published in peer reviewed journal
Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to

Last updated on: 08-11-2019 - 14:12

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: History of use, Internally validated, Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells

Last updated on: 08-11-2019 - 13:59

Contact: Aurélie Crabbé
Organisation: Ghent University
Status: Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of

Last updated on: 08-11-2019 - 13:58

Contact: Tom Coenye
Organisation: Ghent University
Status: Published in peer reviewed journal