A compound of interest (e.g. new psychoactive substance, endocrine disrupting compound, ...) is incubated with human liver microsomes and liver cytosolic fractions to generate both Phase I and II metabolites. Samples are prepared for analysis using a simple method in order to avoid possible losses

Last updated on: 08-11-2019 - 14:01

Organisation: University of Antwerp
Status: Published in peer reviewed journal
Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes

Last updated on: 08-11-2019 - 14:00

Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells

Last updated on: 08-11-2019 - 13:59

Contact: Aurélie Crabbé
Organisation: Ghent University
Status: Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of

Last updated on: 08-11-2019 - 13:58

Contact: Tom Coenye
Organisation: Ghent University
Status: Published in peer reviewed journal
The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.

Last updated on: 08-11-2019 - 13:57

Contact: Tom Coenye
Organisation: Ghent University
Status: Published in peer reviewed journal
ProDigest's short-term colonic simulation is an in vitro model for rapid screening of the interaction between test products and the gut microbiome. The key microbial saccharolytic and proteolytic markers are analysed as well as evolution in the composition of the microbiome and other endpoints as

Last updated on: 08-11-2019 - 13:57

Contact: Aurélien Baudot
Organisation: ProDigest
Partners: ProDigest, ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
Hyper Polarized (HP) MR allows to considerably increase the sensitivity (>10.000) of MR spectroscopy and spectroscopic imaging. The hyperpolarized molecule can be injected at room temperature to a cell system or an in vivo model and its metabolism can be followed over a few minutes.The technique

Last updated on: 08-11-2019 - 13:56

Organisation: UCLouvain
Status: Published in peer reviewed journal
This method evaluates sprouting propensity of endothelial cells in a collagen matrix. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts that form on endothelial spheroids. Endothelial spheroids are obtained by growing

Last updated on: 08-11-2019 - 13:47

Organisation: Ghent University
Status: Internally validated
HR MAS analysis of intact tissues consists in the metabolic profiling of entire tumor biopsies using high resolution NMR (nuclear magnetic resonance) using 1H and/or 13C MRS (magnetic resonance spectroscopy). The technique allows combination of metabolomic data with genomic or proteomic data, and

Last updated on: 08-11-2019 - 13:39

Organisation: UCLouvain
Status: History of use, Published in peer reviewed journal
In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.

Last updated on: 08-11-2019 - 13:37

Contact: Surajit Sahu
Organisation: Vrije Universiteit Brussel
Status: History of use, Published in peer reviewed journal