Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and

Last updated on: 31-01-2023 - 15:52

Contact: Bella Manshian
Organisation: Katholieke Universiteit Leuven (KUL)
This method describes the generation of porcine testicular organoids using piglet testicular cells seeded in a testicular extracellular matrix (tECM) hydrogel. To generate the solublized tECM hydrogel, porcine immature testicular tissues (ITTs) were dissected in small fragments and decellularized in

Last updated on: 04-11-2022 - 08:20

Contact: Marc Kanbar
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are

Last updated on: 07-11-2022 - 11:24

Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal
These are the general guidelines on sample input requirements to successfully start our RIBO-seq procedures: Cell lines in suspension: pellet of 10 – 50 M snap frozen cells. The pre-treatment and collection specifications are also mentioned in our pre-processing protocol. Adherent cell lines

Last updated on: 21-10-2022 - 10:33

Contact: Zarel Martinez
Organisation: OHMX.bio
Status: History of use, Internally validated, Published in peer reviewed journal
We have developed a protocol for culturing primary neuron progenitor cells (NPCs) derived from mouse embryos at embryonic day 15 (E15). These cells are highly proliferative, can be subcultured and cryopreserved and can be differentiated to neurons or astrocytes. Hence, this method can greatly reduce

Last updated on: 20-10-2022 - 08:55

Contact: Roel Quintens
Organisation: Belgian Nuclear Research Centre
Status: Still in development, History of use, Internally validated
The protocol is aimed at developing primary mammary gland organoids that have a morphology similar to the one of the in vivo breast, which is organized as a complex network of interconnected branches. The organoids are derived from the mouse mammary gland by mechanical dissociation and enzymatic

Last updated on: 07-11-2022 - 11:08

Organisation: Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
This method is used to let cells interact for a better simulation of processes occurring in the body. The rational of the method is that monoculture experiments do not capture the complexity of in vivo interactions between different organs. In the most simple setup (published work Nutrients), Caco-2

Last updated on: 09-02-2023 - 16:37

Contact: Amar van Laar
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The mammary-specific polyomavirus middle T antigen overexpression mouse model (MMTV-PyMT) is one of the most commonly used models in the cancer research field for multiple reasons, among which the spontaneous development of multifocal luminal tumors, the early tumoral onset, and the primary tumors'

Last updated on: 07-11-2022 - 11:47

Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
ZeptoWard is a Machine Learning solution (AI) which identifies the ADMET properties of compounds. It can accurately predict over 80 properties related to absorption, distribution, metabolism, excretion, and toxicity properties, how a specific compound or combination of compounds will perform. It can

Last updated on: 21-03-2023 - 13:34

Contact: Segolene Martin
Organisation: Kantify
Status: Published in peer reviewed journal