This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 02-04-2019 - 10:45

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel
Status: Still in development

The monocyte-activation test (MAT) is used to detect or quantify substances that activate human monocytes or monocytic cells to release endogenous mediators such as pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6).

Last updated on: 27-03-2019 - 16:50

Contact: Celine Vanhee
Organisation: Sciensano
Status: Still in development, History of use

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as an hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular responses in a

Last updated on: 27-03-2019 - 16:43

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human

Last updated on: 22-03-2019 - 11:39

Organisation: Vrije Universiteit Brussel
Status: Still in development, History of use

Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells

Last updated on: 22-03-2019 - 11:39

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation

Last updated on: 22-03-2019 - 11:37

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel
Status: Still in development

The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP) [1],[2]. In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In

Last updated on: 22-03-2019 - 11:37

Organisation: Vrije Universiteit Brussel
Status: Still in development

Polycaprolactone (PCL) fiber materials are fabricated using an electrospinning method [1]. Molecular weight of PCL is 45 000 Da (Sigma-Aldrich). The electrospinning process is performed using 18wt% PCL solution dissolved in chloroform:ethanol at a ratio of 9:1. Two high-voltage sources are used

Last updated on: 22-03-2019 - 11:36

Organisation: Vrije Universiteit Brussel
Status: History of use