In this method, we use rabbit monoclonal antibodies that are produced in vitro to identify xenografted human stem cell-derived hepatocytes in frozen murine liver slices. These recombinant antibodies were produced in vitro by cloning antibody genes for immune-specific heavy and light antibody chains

Last updated on: 28-02-2022 - 11:02

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Cultivated liver cells are fixed with 4% (w/v) paraformaldehyde (PFA) for 10 minutes at room temperature and subsequently incubated for 15 minutes with 100 mM glycin solution, used to saturate reactive groups generated after PFA fixation. These cells are subsequently incubated for 10 minutes with 1%

Last updated on: 28-02-2022 - 11:02

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Rat liver epithelial cells are cultivated at 100% confluency on 100 µg/mL rat tail collagen type I coated culture dishes in base medium and sequentially exposed to hepatogenic growth factors and cytokines. Base medium consisted of William’s E medium without glutamine supplemented with 7.33 IE/mL

Last updated on: 28-02-2022 - 11:02

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Rat liver epithelial cells (rLEC) can be isolated from 8-day old male Sprague-Dawley rats. Briefly, small fragments of neonatal rat livers are incubated for 15 minutes with 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES) buffered trypsin solution [0.25% (v/v)] and washed twice with

Last updated on: 28-02-2022 - 11:02

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Mononuclear cells (MNC) are isolated from bone marrow aspirates by density gradient centrifugation and washed in Hank's buffered salt solution. MNC are seeded at a cell density of 2 × 10E4 cells/cm2 in low glucose DMEM supplemented with 15% (v/v) heat-inactivated FBS, 2 mM L-glutamine and 0.5% (v/v)

Last updated on: 28-02-2022 - 11:02

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Approximately 125 g of processed adipose tissue is incubated for 90 minutes at 37°C in dissociation medium (1:1) consisting of 1% (v/v) bovine serum albumin and 1 mg/mL collagenase A in phosphate buffered saline (PBS). After two filtration steps, the filtrate is carefully brought on top of 15 mL of

Last updated on: 28-02-2022 - 11:01

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Human skin-derived precursor cells (hSKP) between are cultivated at 90% confluency on 100 µg/mL collagen type I in base medium and sequentially exposed to hepatogenic factors. Base medium consists of Dulbecco’s Modified Eagle Medium + GLUTAMAX / F12 Nutrient supplement (3:1), supplemented with 7.33

Last updated on: 28-02-2022 - 11:01

Contact: Cannot be disclosed
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
Freshly collected human foreskin samples are incubated with 0.2 mg/mL Liberase DH solution and incubated overnight at 4°C. The next day, the epidermis is removed and the tissue is incubated at 37°C for another 10-20 minutes, depending on the sample size. After processing the samples, typically 5 -

Last updated on: 28-02-2022 - 11:01

Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
This method is a straightforward, colorimetric, and inexpensive high-throughput screening system in bacteria which depends on the activity of human HPD. This screening assay is based on the formation and accumulation of a melanin-like ochronotic pigment which has a characteristic brown color. In the

Last updated on: 28-02-2022 - 11:01

Organisation: Vrije Universiteit Brussel (VUB), RWTH Aachen
Partners: RWTH Aachen
Status: Published in peer reviewed journal
E. coli is one of the organisms of choice for the production of recombinant proteins. DH5 alpha cells are commonly used for maintenance, propagation and mutation, whilst BL21(DE3) and C43(DE3) are mainly used for expression of the transgene. The advantage of C43(DE3) is that is used to produce

Last updated on: 28-02-2022 - 11:01

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use, Internally validated