This method evaluates sprouting propensity of endothelial cells in a collagen matrix. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts that form on endothelial spheroids. Endothelial spheroids are obtained by growing

Last updated on: 08-11-2019 - 13:47

Organisation: Ghent University
Status: Internally validated

HR MAS analysis of intact tissues consists in the metabolic profiling of entire tumor biopsies using high resolution NMR (nuclear magnetic resonance) using 1H and/or 13C MRS (magnetic resonance spectroscopy). The technique allows combination of metabolomic data with genomic or proteomic data, and

Last updated on: 08-11-2019 - 13:39

Organisation: UCLouvain
Status: History of use, Published in peer reviewed journal

In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.

Last updated on: 08-11-2019 - 13:37

Contact: Surajit Sahu
Organisation: Vrije Universiteit Brussel
Status: History of use, Published in peer reviewed journal

Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.

Last updated on: 08-11-2019 - 13:36

Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal

Sandwich-cultured hepatocytes are exposed to test compounds (e.g. drug candidates) or known hepatotoxicants (as controls) both in the absence and in the presence of a mixture of physiologically relevant bile acids. After 24h, decreased hepatocyte viability and functionality in the presence of

Last updated on: 08-11-2019 - 13:35

Contact: Pieter Annaert
Organisation: KU Leuven
Status: Internally validated, Published in peer reviewed journal

This method is used to monitor cardiac cell cultures in vitro. It can be used for monitoring both cell activity and drug-induced cardiac side-effects for primary cardiomyocytes or hIPSC-derived cardiomyocytes. Unlike conventional microelectrode array systems that only measure the local

Last updated on: 08-11-2019 - 13:34

Contact: Thomas Pauwelyn
Organisation: IMEC
Status: Published in peer reviewed journal

Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and

Last updated on: 08-11-2019 - 13:33

Organisation: Vrije Universiteit Brussel
Status: Still in development

Despite major advances in our understanding of neurodegenerative disorders, no efficient therapies are available for patients with dementia, motor neuron disease and other neurodegenerative disorders. With the advent of pluripotent stem cells (PSCs) it now becomes possible to better model human

Last updated on: 08-11-2019 - 13:32

Contact: Cannot be disclosed
Organisation: KU Leuven
Status: Still in development, Internally validated

We developed in vitro models to study liver disease, such as liver inflammation and fibrosis, as seen in non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH); or hepatitis viral infection; or to enhance our ability to detect drugs that cause acute or repeat dose

Last updated on: 08-11-2019 - 13:32

Contact: Cannot be disclosed
Organisation: KU Leuven
Status: Internally validated, Published in peer reviewed journal

The chorioallantoic membrane (CAM) is a highly vascularized membrane which results from the fusion of two extra-embryonic membranes, namely the chorion and the allantois. The CAM is easily accessible for manipulation and imaging, but methodologies differ whether the chicken embryo stays within

Last updated on: 08-11-2019 - 13:27

Contact: Hanna Tay
Organisation: Ghent University
Status: Internally validated