The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.

Last updated on: 08-11-2019 - 13:57

Contact: Tom Coenye
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
ProDigest's short-term colonic simulation is an in vitro model for rapid screening of the interaction between test products and the gut microbiome. The key microbial saccharolytic and proteolytic markers are analysed as well as evolution in the composition of the microbiome and other endpoints as

Last updated on: 08-11-2019 - 13:57

Contact: Aurélien Baudot
Organisation: ProDigest
Partners: ProDigest
Status: History of use, Internally validated, Published in peer reviewed journal
Hyper Polarized (HP) MR allows to considerably increase the sensitivity (>10.000) of MR spectroscopy and spectroscopic imaging. The hyperpolarized molecule can be injected at room temperature to a cell system or an in vivo model and its metabolism can be followed over a few minutes.The technique

Last updated on: 08-11-2019 - 13:56

Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
This method evaluates sprouting propensity of endothelial cells in a collagen matrix. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts that form on endothelial spheroids. Endothelial spheroids are obtained by growing

Last updated on: 08-11-2019 - 13:47

Organisation: Ghent University (UGent)
Status: Internally validated
HR MAS analysis of intact tissues consists in the metabolic profiling of entire tumor biopsies using high resolution NMR (nuclear magnetic resonance) using 1H and/or 13C MRS (magnetic resonance spectroscopy). The technique allows combination of metabolomic data with genomic or proteomic data, and

Last updated on: 08-11-2019 - 13:39

Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Published in peer reviewed journal
Sandwich-cultured hepatocytes are exposed to test compounds (e.g. drug candidates) or known hepatotoxicants (as controls) both in the absence and in the presence of a mixture of physiologically relevant bile acids. After 24h, decreased hepatocyte viability and functionality in the presence of bile

Last updated on: 08-11-2019 - 13:35

Contact: Pieter Annaert
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Internally validated, Published in peer reviewed journal
This method is used to monitor cardiac cell cultures in vitro. It can be used for monitoring both cell activity and drug-induced cardiac side-effects for primary cardiomyocytes or hIPSC-derived cardiomyocytes. Unlike conventional microelectrode array systems that only measure the local field

Last updated on: 08-11-2019 - 13:34

Contact: Thomas Pauwelyn
Organisation: Interuniversitair Micro-Electronica Centrum (IMEC)
Status: Published in peer reviewed journal
Despite major advances in our understanding of neurodegenerative disorders, no efficient therapies are available for patients with dementia, motor neuron disease and other neurodegenerative disorders. With the advent of pluripotent stem cells (PSCs) it now becomes possible to better model human

Last updated on: 08-11-2019 - 13:32

Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated
We developed in vitro models to study liver disease, such as liver inflammation and fibrosis, as seen in non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH); or hepatitis viral infection; or to enhance our ability to detect drugs that cause acute or repeat dose drug induced

Last updated on: 08-11-2019 - 13:32

Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Internally validated, Published in peer reviewed journal
The chorioallantoic membrane (CAM) is a highly vascularized membrane which results from the fusion of two extra-embryonic membranes, namely the chorion and the allantois. The CAM is easily accessible for manipulation and imaging, but methodologies differ whether the chicken embryo stays within its

Last updated on: 08-11-2019 - 13:27

Contact: Hanna Tay
Organisation: Ghent University (UGent)
Status: Internally validated