Mononuclear cells are flushed from the bone marrow using a 25G needle. After centrifugation, the cells are resuspended in 50mL pre-heated DMEM containing 20ng/ml M-CSF. The cells are cultured in 10cm petri dishes for 7 days. At days 2,4 and 6, the medium is renewed after washing away non-adherent

Last updated on: 10-03-2020 - 10:17

Contact: Cannot be disclosed
Organisation: Ghent University
Status: Published in peer reviewed journal
The method is an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells. Naive CD8+ T cells are isolated from mouse

Last updated on: 05-03-2020 - 14:59

Contact: Dorien Broekaert
Organisation: KU Leuven, VIB KU Leuven
Status: Published in peer reviewed journal
Organotypic epithelial raft cultures accurately reproduce the process of epithelial differentiation in vitro and can be prepared from normal keratinocytes, explanted epithelial tissue, or established cell lines. Normal primary human keratinocytes (PHKs) stratify and fully differentiate in a manner

Last updated on: 04-03-2020 - 14:34

Contact: Graciela Andrei
Organisation: KU Leuven
Status: History of use, Internally validated, Published in peer reviewed journal
This white-box model uses energy partitioning throughout the lifetime of dairy animals (growth, lactation, gestation, ...) to simulate reproduction performance, lifetime length, production performance etc. The method is developed by dr. Olivier Martin at INRAE, MoSAR, Paris.

Last updated on: 04-03-2020 - 14:23

Contact: Cannot be disclosed
Organisation: Cannot be disclosed
Status: History of use, Published in peer reviewed journal
Brain from treated and not treated mice is collected after death. Tissue is fixed in 4% PFA for 4 days. After specific cutting (ex. L and R hemisphere) tissue is placed in a cassette. Cassette is placed in Tissue Processor (where water from the tissue is removed and replaced with paraffin). Brain is

Last updated on: 04-03-2020 - 14:20

Contact: Cannot be disclosed
Organisation: KU Leuven, KU Leuven
Status: History of use, Internally validated
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell

Last updated on: 03-03-2020 - 11:46

Contact: Cannot be disclosed
Organisation: Hasselt University
Status: Published in peer reviewed journal
Since adult zebrafish retinal ganglion cells (RGCs) can fully regenerate upon axonal injury, these neurons form the ideal subject to study what is driving the recovery process. The use of an adult zebrafish retinal cell culture in a microfluidic set-up enables to create a neuronal network, mimicking

Last updated on: 02-03-2020 - 16:01

Organisation: KU Leuven
Status: Still in development
This is a method to study host-microbe interaction in the upper respiratory tract. A physiologically representative epithelial structure, with mucin producing and ciliated cells, is obtained by culturing respiratory epithelial cells at air-liquid interface in Transwell inserts. Optionally,

Last updated on: 28-02-2020 - 10:51

Organisation: Ghent University
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal
We use stem cell-derived sensory neurons and via a small molecule-based protocol (Young et al, 2014) we derive them into human sensory neurons with a nociceptor phenotype (express somatosensory ion channels).

Last updated on: 28-02-2020 - 10:49

Contact: Cannot be disclosed
Organisation: Cannot be disclosed
Status: Still in development, Published in peer reviewed journal
We propose to generate three-dimensional tumoroids from the primary melanoma cell lines, as well as 3D organoids from the iPSc-derived neural crest stem cells. We will use the AggreWell system (STEMCELL Technologies) to generate uniform, size-controlled three-dimensional spheroids. After 5 days in

Last updated on: 25-02-2020 - 23:58

Organisation: VIB KU Leuven
Status: Published in peer reviewed journal