The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as
Last updated on: 16-08-2022 - 10:57
Contact: Marie-Isabelle Garcia
Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
DARTpaths is an an integrative app to support the prioritisation of chemicals. The Open Source R shiny application allows for the prediction of compound-induced molecular mechanisms of action. The tool integrates phenotypic endpoints of different species induced by compounds and genetic variants, in
Last updated on: 13-06-2022 - 16:04
Contact: Vera van Noort
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Open Analytics, Hogeschool Utrecht , Vivaltes
Status: Internally validated
In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on
Last updated on: 26-05-2022 - 10:41
Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination
Last updated on: 18-02-2022 - 16:42
Contact: Ha Nguyen
Organisation: Université Libre de Bruxelles (ULB), Sciensano
Partners: University of Liège (ULiège)
Status: History of use, Internally validated
Quantum Dots (QDs) have been hypothesized as potential probes for the optical monitoring of electrogenic cell activity given their high optical absorption cross-sections compared to organic fluorophores, high brightness and low photobleaching. Despite the theoretical predictions, less than a handful
Last updated on: 04-08-2021 - 16:50
Contact: Stijn Jooken
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We used a 7-stage protocol to generate beta cells from human Induced Pluripotent Stem Cells (iPSC) and evaluated whether these cells are responsive to the pro-inflammatory cytokines (IFNγ, IL-1β, or IFNα) that play a role in type 1 diabetes (T1D). Our data show that human iPSC-derived beta cells
Last updated on: 09-03-2021 - 21:30
Contact: Decio L. Eizirik
Organisation: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
We have established organoid cultures from patient-derived ovarian cancer (OC), in particular from the most prevalent high-grade serous ovarian cancer (HGSOC). Testing multiple culture medium components identified neuregulin-1 (NRG1) as key factor in maximizing OC organoid development and growth,
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have developed multiple organoid models from a broad spectrum of endometrial pathologies that capture endometrial disease diversity and will provide powerful research models and drug screening and discovery tools. Organoids from endometriosis show disease-associated traits and cancer-linked
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We have established organoids from mouse pituitary with the aim to generate a novel research model to study pituitary stem cell biology in both healthy and diseased glands. The organoids originated from the pituitary cells expressing the stem cell marker SOX2, were long-term expandable, displayed a
Last updated on: 28-01-2021 - 11:08
Contact: Hugo Vankelecom
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We tissue-engineer in vitro, skeletal muscle consisting of aligned myofibers. To create the so-called bio-artificial muscle (BAM), human muscle progenitor cells are expanded, and a 3D construct is created by mixing the cells with a hydrogel. The cell-gel mix is cast into custom-made silicone molds
Last updated on: 08-12-2020 - 17:46
Contact: Lieven Thorrez
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
