The mammary-specific polyomavirus middle T antigen overexpression mouse model (MMTV-PyMT) is one of the most commonly used models in the cancer research field for multiple reasons, among which the spontaneous development of multifocal luminal tumors, the early tumoral onset, and the primary tumors'

The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are

The protocol is aimed at developing primary mammary gland organoids that have a morphology similar to the one of the in vivo breast, which is organized as a complex network of interconnected branches. The organoids are derived from the mouse mammary gland by mechanical dissociation and enzymatic

A battery of in vitro genotoxicity assays is currently used to detect agents with DNA damaging and carcinogenic potential. Although the sensitivity of this battery of genotoxicity assays is high, the specificity is low (“false positive hit”), especially with the mammalian cell-based assay, compared