The use of three-dimensional (3D) cell culture models is considered a reliable model that is successfully used for high-throughput drug testing in carcinoma research. However, the development of soft tissue sarcoma organoids is lagging behind. Soft tissue sarcoma is a group of rare malignancies from
Last updated on: 30-07-2024 - 12:08
Contact: Agnieszka Wozniak
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Still in development
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that
Last updated on: 23-11-2022 - 16:31
Contact: Birgit Mertens
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The mammary-specific polyomavirus middle T antigen overexpression mouse model (MMTV-PyMT) is one of the most commonly used models in the cancer research field for multiple reasons, among which the spontaneous development of multifocal luminal tumors, the early tumoral onset, and the primary tumors'
Last updated on: 07-11-2022 - 11:47
Contact: Beaunelle de Bruijn
Organisation: Oncology - KU Leuven, Katholieke Universiteit Leuven (KUL)
The protocol is aimed at developing primary human breast organoids that have a morphology similar to the one observed in the in vivo breast. This morphology encompasses a complex network organization composed of interconnected branches that terminate in TDLU-like structures. The organoids are
Last updated on: 07-11-2022 - 11:24
Contact: Beaunelle de Bruijn
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: Still in development, History of use, Published in peer reviewed journal
The protocol is aimed at developing primary mammary gland organoids that have a morphology similar to the one of the in vivo breast, which is organized as a complex network of interconnected branches. The organoids are derived from the mouse mammary gland by mechanical dissociation and enzymatic
Last updated on: 07-11-2022 - 11:08
Contact: Beaunelle de Bruijn
Organisation: Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
The VITOTOX test is a bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controlled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After incubation
Last updated on: 26-05-2022 - 10:40
Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The CALUX® system (Chemically Actived LUciferase eXpression) of Bio Detection Systems (BDS, Amsterdam, the Netherlands) uses U-2 OS cells (human osteoblast) that are stably transfected with human TRb or human PPARg2 (BDS, Amsterdam) and a luciferase reporter construct under the control of a receptor
Last updated on: 05-05-2022 - 08:59
Contact: Cannot be disclosed
Organisation: Sciensano
Partners: BDS
Status: Published in peer reviewed journal
In silico tools are computer-assisted methodologies with a high-throughput that allow to predict the toxic potential of compounds without experimental testing. Consequently, in silico tools are time-, cost- and animal-saving in nature. The most commonly used methods are (quantitative) structure
Last updated on: 24-03-2022 - 11:25
The ATP cell viability assay (CellTiter-Glo assay test kit) provides a quantitative measurement of the ATP content directly proportional to the number of cells present in culture. The homogeneous assay procedure involves adding one single reagent, CellTiter-Glo Reagent directly to cells cultured in
Last updated on: 24-03-2022 - 11:20
Contact: Cannot be disclosed
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The tetanus neurotoxin (TeNT) is one of the most toxic proteins known to man, which prior to the use of the vaccine against the TeNT producing bacteria Clostridium tetani, resulted in a 20% mortality rate upon infection. The clinical detrimental effects of tetanus have decreased immensely since the
Last updated on: 22-03-2022 - 16:49