Cellular / Slice electrophysiology
Scope of the method
- Human health
- Basic Research
- In vitro - Ex vivo
- Animal derived cells / tissues / organs
- Patch clamp
- Field potential
- Brain slice
In this method, it is possible to use active/viable animal or human brain slices / cells (normal or disease model) to study the effects of different drugs on brain cells (neuron or glia) in diverse brain region.
- Vibration isolation table with Faraday cage ;
- Signal amplifier ;
- Digitizer ;
- Micromanipulator ;
- IR-DIC Microscope ;
- Perfusion pump ;
- Glass pipette microforge.
- History of use
- Published in peer reviewed journal
Pros, cons & Future potential
- Reduce animal use ;
- Any drugs can be tested before clinical studies ;
- Possibility of sharing same animal for different experiments depending on target brain regions.
- Require sophisticated instruments which are expensive ;
- Require long training before successful implementation.
References, associated documents and other information
Fanny Sandrine Martineau, Surajit Sahu, Vanessa Plantier, Emmanuelle Buhler, Fabienne Schaller, Lauriane Fournier, Geneviève Chazal, Hiroshi Kawasaki, Alfonso Represa, Françoise Watrin, Jean-Bernard Manent, Correct Laminar Positioning in the Neocortex Influences Proper Dendritic and Synaptic Development, Cerebral Cortex, Volume 28, Issue 8, August 2018, Pages 2976–2990, https://doi.org/10.1093/cercor/bhy113
Contact personSurajit Sahu
OrganisationsVrije Universiteit Brussel (VUB)
Pharmaceutical and Pharmacological Sciences