We devised a simple and reliable method for reprogramming peripheral blood mononuclear cells into hiPSC and then to differentiate them into air-liquid interface bronchial epithelium (iALI) within 45 days. Of note, this method does not involve any cell sorting step. We reprogrammed blood cells from

Last updated on: 07-02-2023 - 14:46

Contact: Engi Ahmed
Organisation: VIB - UGent, IRMB INSERM 1183
Status: History of use, Internally validated, Published in peer reviewed journal
Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes. Despite the big burden, there is no effective treatment to restore tendon’s natural composition, due to a lack of understanding of fundamental cell biology. Additionally, translation of novel

Last updated on: 17-01-2023 - 16:22

Organisation: Ghent University (UGent)
Status: Still in development
Using the ex vivo culture system, we investigated the impact of an endoscopic helium plasma jet application on mouse ISCs at the morphological, cellular and transcriptomic levels. Moreover, we explored the potential selectivity of CAP application on tumor versus normal organoids originating from the

Last updated on: 09-01-2023 - 09:42

Organisation: Université Libre de Bruxelles (ULB)
Status: Published in peer reviewed journal
This study aimed to create a versatile and reproducible in vitro model of a human motor unit with functional neuromuscular junctions (NMJs). Therefore, human induced pluripotent stem cell (hiPSC)-derived motor neurons and human primary mesoangioblast (MAB)-derived myotubes were co-cultured in

Last updated on: 09-01-2023 - 15:13

Organisation: Vlaams Instituut voor Biotechnologie (VIB), Katholieke Universiteit Leuven (KUL), Neurosciences - KU Leuven
Status: Published in peer reviewed journal
Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the

Last updated on: 10-01-2023 - 16:48

Contact: Yani Berckmans
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are

Last updated on: 13-12-2022 - 11:23

Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
Alkaptonuria (AKU) is a rare inborn error of metabolism caused by a defective homogentisate 1,2-dioxygenase (HGD), an enzyme involved in the tyrosine degradation pathway. Loss of HGD function leads to the accumulation of homogentisic acid (HGA) in connective body tissues in a process called

Last updated on: 06-12-2023 - 14:45

Contact: Sien Lequeue
Organisation: Vrije Universiteit Brussel (VUB)
Partners: RWTH Aachen
Status: Published in peer reviewed journal
This method relates to the development of highly reproducible human iPSC-derived neurospheroids equipped with intrinsic bioluminescence for an easy and longitudinal follow-up of the viability and growth of these neurospheroids over time. The luminescent neurospheroids have been applied in ischemic

Last updated on: 02-01-2023 - 10:27

Organisation: University of Antwerp (UAntwerpen)
Status: Published in peer reviewed journal
Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and

Last updated on: 31-01-2023 - 15:52

Contact: Bella Manshian
Organisation: Katholieke Universiteit Leuven (KUL)
This method describes the generation of porcine testicular organoids using piglet testicular cells seeded in a testicular extracellular matrix (tECM) hydrogel. To generate the solublized tECM hydrogel, porcine immature testicular tissues (ITTs) were dissected in small fragments and decellularized in

Last updated on: 04-11-2022 - 08:20

Contact: Marc Kanbar
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal