Freshly collected human foreskin samples are incubated with 0.2 mg/mL Liberase DH solution and incubated overnight at 4°C. The next day, the epidermis is removed and the tissue is incubated at 37°C for another 10-20 minutes, depending on the sample size. After processing the samples, typically 5 -
Last updated on: 28-02-2022 - 11:01
Contact: Joery De Kock
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
This method is a straightforward, colorimetric, and inexpensive high-throughput screening system in bacteria which depends on the activity of human HPD. This screening assay is based on the formation and accumulation of a melanin-like ochronotic pigment which has a characteristic brown color. In the
Last updated on: 28-02-2022 - 11:01
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB), RWTH Aachen
Partners: RWTH Aachen
Status: Published in peer reviewed journal
E. coli is one of the organisms of choice for the production of recombinant proteins. DH5 alpha cells are commonly used for maintenance, propagation and mutation, whilst BL21(DE3) and C43(DE3) are mainly used for expression of the transgene. The advantage of C43(DE3) is that is used to produce
Last updated on: 28-02-2022 - 11:01
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use, Internally validated
HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human cells
Last updated on: 28-02-2022 - 11:01
Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use
This method describes the steps to go from a liver to a decellularized matrix. It uses mild and strong detergents to destroy cells and keep the extracellular matrix intact. This matrix can then be used for a variety of purposes, including (but not limited to) repopulation, basis for coating and
Last updated on: 28-02-2022 - 11:01
Contact: Paul Claes
Organisation: Vrije Universiteit Brussel (VUB)
Status: Published in peer reviewed journal
This method describes the steps from a living rat to a single cell solution of primary hepatocytes. This requires surgery on the lab animal, a perfusion with buffer solution, a digestion with collagenase and a filtration step to obtain primary hepatocytes.
Last updated on: 28-02-2022 - 11:01
This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.
Last updated on: 28-02-2022 - 11:00
Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417
Last updated on: 28-02-2022 - 11:00
Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated
Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells exhibit a
Last updated on: 28-02-2022 - 11:00
Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation occurs
Last updated on: 28-02-2022 - 11:00
Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
