Functional cardiomyocytes can be efficiently derived from human pluripotent stem cells, which collectively include embryonic and induced pluripotent stem cells (iPSC). Specific affected biological pathways involved in disease can be functionally studied in differentiated cells at a single patient

Last updated on: 08-11-2019 - 11:01

Organisation: Ghent University
Status: Internally validated

The Koken rat is a dummy rat that is developed to train animal procedures, such as handling, blood sampling and intubation.

Last updated on: 08-11-2019 - 10:24

Contact: Cannot be disclosed
Organisation: Cannot be disclosed

The DPRA is an in chemico method which quantifies the remaining concentration of cysteine- or lysine-containing peptide following 24 hours incubation with the test chemical at 25 +/-2,5ºC. The synthetic peptides contain phenylalanine to aid in the detection. Relative peptide

Last updated on: 08-11-2019 - 10:21

Contact: bart Desmedt
Organisation: Sciensano
Partners: Sciensano
Status: History of use, Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)

The monocyte-activation test (MAT) is used to detect or quantify substances that activate human monocytes or monocytic cells to release endogenous mediators such as pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6).

Last updated on: 08-11-2019 - 10:13

Contact: Celine Vanhee
Organisation: Sciensano
Status: Still in development, History of use

HepG2 is a human hepatoma derived cell line, which are epithelial in morphology. It was established from liver tissue of a 15-year-old Caucasian male with a well differentiated hepatocellular carcinoma. The HepG2 cell line is one of the most used human liver-based in vitro models. The

Last updated on: 08-11-2019 - 10:11

Contact: Anja Heymans
Organisation: Vrije Universiteit Brussel
Status: History of use

Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) express several key liver-specific transcription factors as well as hepatic progenitor markers. However, they still lack the hepatocyte nuclear factors 1-alpha (HNF1a) and 4-alpha (HNF4a), indispensable for their reprogramming

Last updated on: 08-11-2019 - 10:09

Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel
Status: Still in development

Albumin secretion in the supernatant of in vitro cultures can be measured by an enzyme-linked immunosorbent assay. The assay is based upon a quantitative sandwich enzyme immunoassay technique and measures albumin in the culture medium of primary hepatocytes and/or hepatocyte like cells.

Last updated on: 08-11-2019 - 09:52

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use

Liver functionality can be monitored by the urea synthesis. In culture medium the measurement of urea synthesis relies on a chromogenic reagent that specifically forms a colored complex with urea. The concentration of this complex between urea, o-phthalaldehyde and N-(1-naphthyl) ethylenediamine

Last updated on: 08-11-2019 - 09:49

Contact: Steven Branson
Organisation: Vrije Universiteit Brussel
Status: History of use

This method uses human skin-derived precursors (hSKP) differentiated towards hepatic cells (hSKP-HPC) as a hepatic in vitro model. Exposure of these cells for 24 hours to sub-cytotoxic concentrations of acetaminophen, which is a reference hepatotoxicant, induced specific cellular

Last updated on: 08-11-2019 - 09:48

Contact: Robim Rodrigues
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal

This method provides a new and easy to standardize enzymatic isolation protocol to obtain human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs). hUC-MSCs are obtained within 3 hours and the isolation method provides a minimal risk of bacterial contamination. The so-obtained hUC-MSCs

Last updated on: 08-11-2019 - 09:46

Contact: Karolien Buyl
Organisation: Vrije Universiteit Brussel
Status: Published in peer reviewed journal