Generally, our research group aims to study the influence of food parameters (food design, food processing, food composition) on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use in vitro digestion protocols. The current method is a semi
Last updated on: 15-02-2023 - 16:08
Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and
Last updated on: 31-01-2023 - 15:52
Human Intestinal Organoids (HIOs) are in vitro 3D cell cultures arranged in a crypt-villus structure that incorporate many physiological features of the intestinal epithelium, including the presence of different cell populations (enterocytes, goblet cells, enteroendocrine and Paneth cells). HIOs can
Last updated on: 27-01-2023 - 13:29
Contact: Joana Rocha-Pereira
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of
Last updated on: 11-01-2023 - 16:43
Contact: Bram Verstockt
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the
Last updated on: 10-01-2023 - 16:48
Contact: Yani Berckmans
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
For therapeutic or reporter molecules to be effective for therapy or imaging applications, proper accumulation of the compounds in the tissue of interest is required, with minimal accumulation in undesired organs to avoid toxic side-effects and increase bioavailability. After initial in vitro
Last updated on: 09-08-2022 - 16:34
In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on
Last updated on: 26-05-2022 - 10:41
Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
Lung cancer thrives in a complex multicellular tumor microenvironment (TME) that impacts tumor growth, metastasis, response, and resistance to therapy. While orthotopic murine lung cancer models can partly recapitulate this complexity, they do not resonate with high-throughput immunotherapeutic drug
Last updated on: 16-03-2022 - 14:53
Contact: Cleo Goyvaerts
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Institut Curie
Status: Published in peer reviewed journal
This model is a 3D human co-culture model where both hepatocyte functionality and HSC quiescence can be maintained for at least 21 days. This novel system allows hepatotoxicity testing and can detect drug-induced as well as hepatocyte-dependent HSC activation, thereby representing an important step
Last updated on: 16-03-2022 - 14:51
Generation of Hepatic Stellate Cells from Human Pluripotent Stem for in vitro liver fibrosis studies
We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance
Last updated on: 16-03-2022 - 14:49
Contact: Leo A. van Grunsven
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
