Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs
Last updated on: 14-08-2020 - 15:22
Contact: Joy Irobi
Organisation: University of Hasselt (UHasselt)
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
The feed undergoes an in vitro enzymatic hydrolysis simulating the digestion in the upper digestive tract followed by a dialysis mimicking the intestinal absorption. Finally, an in vitro batch fermentation is performed and simulates the fermentation occurring in the hindgut. The ingredients, ground
Last updated on: 16-07-2020 - 15:04
Contact: Nadia Everaert
Organisation: University of Liège (ULiège)
Status: Published in peer reviewed journal
Prebiotics are used by the gut microbiota creating metabolites that exert a positive
effect on the host (postbiotics). Prebiotics are available on the market for human and
animal consumption and may induce differential effects on the host's health and
metabolism. They can be tested by in vitro
Last updated on: 16-07-2020 - 15:02
Contact: Nadia Everaert
Organisation: University of Liège (ULiège)
Status: Published in peer reviewed journal
Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) offer an attractive platform for cardiovascular research, including disease modeling, drug toxicity testing and development of regenerative therapies. Patient-specific iPSC-CMs are very useful to study disease pathogenesis and
Last updated on: 30-06-2020 - 14:13
Contact: Maaike Alaerts
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development, Internally validated
Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.
Last updated on: 03-06-2020 - 13:51
Contact: Aline Verstraeten
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development, Internally validated
Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured
Last updated on: 17-04-2020 - 09:56
Contact: Isabel Beets
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
We study the influence of food parameters on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use a static in vitro digestion protocol based on the standardized, consensus method recommended by the INFOGEST consortium. Briefly, the food is first
Last updated on: 31-03-2020 - 21:01
Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: INFOGEST consortium
Status: Published in peer reviewed journal
The objective of this ex-vivo model is to study the initial pulp-tissue reaction of the human pulp tissue to different pulp-capping materials.
Methodology: Freshly-extracted (mainly due to orthodontic reasons) healthy human teeth (impacted third molars) from young individuals (15-20 years old)
Last updated on: 24-03-2020 - 16:32
Contact: Mariano Pedano
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: Aix-Marseille University
Status: History of use, Internally validated, Published in peer reviewed journal
Retinal gene delivery via intravitreal injection is hampered by various physiological barriers present in the eye of which the vitreoretinal (VR) interface represents the most serious hurdle. We present a retinal explant model especially designed to study the role of this interface as a barrier for
Last updated on: 24-03-2020 - 16:26
Contact: Katrien Remaut
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Design and fabrication of microfluidic devices that allow manipulation and analysis of (single) cells. Droplet-based as well as digital microfluidics can be applied and are suitable for a wide variety of (non-adherent) cells. Different materials can be used for the fabrication of the microfluidic
Last updated on: 10-03-2020 - 14:54
Contact: Cannot be disclosed
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Published in peer reviewed journal
