Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the
Last updated on: 10-01-2023 - 16:48
Contact: Yani Berckmans
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal
The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are
Last updated on: 13-12-2022 - 11:23
Contact: Frédéric Clotman
Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
For the risk assessment of compounds migrating from food contact materials (FCM), information on the exposure to the migrant as well as its possible hazards is needed. To support the evaluation of both starting products and NIAS from plastic FCM, the VERMEER FCM tool has been developed within the
Last updated on: 29-11-2022 - 14:46
This method describes the generation of porcine testicular organoids using piglet testicular cells seeded in a testicular extracellular matrix (tECM) hydrogel. To generate the solublized tECM hydrogel, porcine immature testicular tissues (ITTs) were dissected in small fragments and decellularized in
Last updated on: 04-11-2022 - 08:20
Contact: Marc Kanbar
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
These are the general guidelines on sample input requirements to successfully start our RIBO-seq procedures:
Cell lines in suspension: pellet of 10 – 50 M snap frozen cells. The pre-treatment and collection specifications are also mentioned in our pre-processing protocol.
Adherent cell lines
Last updated on: 21-10-2022 - 10:33
Contact: Zarel Martinez
Organisation: OHMX.bio
Status: History of use, Internally validated, Published in peer reviewed journal
The Koken rat is a dummy rat that is developed to train animal procedures, such as handling, blood sampling and intubation.
Last updated on: 15-10-2022 - 09:06
The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic
Last updated on: 14-10-2022 - 11:37
Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The tri-dimensional (3D) culture protocols allow isolation and culture of gastric epithelial antral and intestinal stem cells to efficiently generate organoids that recapitulate the mature pyloric and intestinal epithelium in vitro and foetal epithelial progenitors of these tissues growing as
Last updated on: 16-08-2022 - 10:57
Contact: Marie-Isabelle Garcia
Organisation: Université Libre de Bruxelles (ULB)
Status: History of use, Internally validated, Published in peer reviewed journal
For therapeutic or reporter molecules to be effective for therapy or imaging applications, proper accumulation of the compounds in the tissue of interest is required, with minimal accumulation in undesired organs to avoid toxic side-effects and increase bioavailability. After initial in vitro
Last updated on: 09-08-2022 - 16:34
The main objective of this research project is to create the first full-thickness cornea-on-chip, which comprises a 3D construct with every cellular layer of the cornea. This construct is embedded in a microfluidic chip with two channels that are continuously perfused. The epithelial side of the
Last updated on: 27-07-2022 - 09:35
Contact: Joris Van Meenen
Organisation: University of Antwerp (UAntwerpen)
Status: Still in development
