Since adult zebrafish retinal ganglion cells (RGCs) can fully regenerate upon axonal injury, these neurons form the ideal subject to study what is driving the recovery process. The use of an adult zebrafish retinal cell culture in a microfluidic set-up enables to create a neuronal network, mimicking
Last updated on: 02-03-2020 - 16:01
Contact: Annelies Van Dyck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development
We use stem cell-derived sensory neurons and via a small molecule-based protocol (Young et al, 2014) we derive them into human sensory neurons with a nociceptor phenotype (express somatosensory ion channels).
Last updated on: 28-02-2020 - 10:49
Contact: Cannot be disclosed
Organisation: Cannot be disclosed
Status: Still in development, Published in peer reviewed journal
We propose to generate three-dimensional tumoroids from the primary melanoma cell lines, as well as 3D organoids from the iPSc-derived neural crest stem cells. We will use the AggreWell system (STEMCELL Technologies) to generate uniform, size-controlled three-dimensional spheroids. After 5 days in
Last updated on: 25-02-2020 - 23:58
Contact: Valerie Christiaens
Organisation: VIB - KU Leuven
Status: Published in peer reviewed journal
Cell line which is commonly used to study biological processes in mouse macrophages. It is a macrophage-like cell line derived from BALB/c mice.
Last updated on: 25-02-2020 - 23:58
Organotypical culture models, such as retinal explants, is an ideal alternative for in vitro retinal cell cultures and preclinical animal models, as they provide the necessary compromise between these two model systems. The major advantage of explant cultures is that cells are kept within their
Last updated on: 21-02-2020 - 14:51
Contact: Evy Lefevere
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Internally validated, Published in peer reviewed journal
We have developed and validated yeast models to study aspects related to protein folding diseases like Alzheimer's and Parkinson's disease. These models allow to gain further insight in the cellular processes involved in the etiology of these disorders and as such identify potential new bio-markers
Last updated on: 11-02-2020 - 11:17
Contact: Joris Winderickx
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The method enables to quantitatively assess the invasion of endothelial cells in extracellular-matrix mimicking hydrogels, such as collagen or polyethylene glycol, and to measure the forces exerted by the cells that enable them to invade. Endothelial cells are seeded on the side of a hydrogel and
Last updated on: 11-02-2020 - 08:55
Contact: Hans Van Oosterwyck
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Patient-derived explants of prostate cancer provide an ex vivo model that retains the architecture and microenvironment of the native tissue. It enables the evaluation of drug responses on individual patient’s tumors ex vivo without passaging in animals. It is compatible with all molecular analysis
Last updated on: 07-02-2020 - 15:55
Contact: Johan Swinnen
Organisation: Katholieke Universiteit Leuven (KUL)
Partners: University of Adelaide
Status: History of use, Published in peer reviewed journal
We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the
Last updated on: 04-02-2020 - 13:31
Contact: Maaike Vercauteren
Organisation: Ghent University (UGent), University of Liège (ULiège)
Status: Internally validated
The GIDM-colon is a validated in vitro model that allows the study of the availability of compounds and the metabolisation at the level of the colon. The human physiological conditions of the gastrointestinal tract (stomach, small intestine and colon) are mimicked. The continiuous flow eliminates
Last updated on: 03-02-2020 - 08:44
Contact: Annelies Breynaert
Organisation: University of Antwerp (UAntwerpen)
Status: History of use, Internally validated, Published in peer reviewed journal
