High-throughput in-flow imaging of intact organoids and tissue (mimics) with cellular resolution

Modern cell and developmental biology increasingly rely on 3D cell systems such as organoids. However, the inability to characterize these specimens at the cellular level with high throughput hampers their integration in routine screening settings. To address this bottleneck, we have developed a module for imaging organoids and tissue (mimics) in flow, based on a transparent agarose fluidic chip that enables efficient and consistent 3D recordings with theoretically unlimited throughput. Downstream high-content image analysis enables absolute quantification with cellular resolution. By design, the platform offers a cost-effective, accessible, and efficient solution for fast and absolute 3D histopathology on essentially any microscope.