Generation of Hepatic Stellate Cells from Human Pluripotent Stem for in vitro liver fibrosis studies

We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance

Last updated on: 16-03-2022 - 14:49

Contact: Leo A. van Grunsven
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
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Chemical Activated LUciferase gene eXpression

The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and

Last updated on: 16-03-2022 - 14:47

Contact: Imke Boonen
Organisation: Vrije Universiteit Brussel (VUB)
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Mouse in vitro follicle culture bioassay for fundamental and translational research on oocyte developmental capacity

Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes

Last updated on: 16-03-2022 - 14:32

Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use, Internally validated, Published in peer reviewed journal
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Fabrication of optofluidic and microfluidic devices

Prototyping and replication (small series production) of microfluidic or optofluidic devices, in thermoplastic polymers or in glass. 3D nanoprinting is also available to produce microscaffolds, possibly within microfluidic channels.

Last updated on: 16-03-2022 - 14:25

Contact: Jürgen Van Erps
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal
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Compact, multimodal spectroscopic devices for the read-out of microfluidic organs-on-chip

Recent improvements on the structural aspects of organ-on-chips pave the way towards a large-scale application. As such soon the number of read-out instruments that are in operation in parallel will need to drastically increase. Unfortunately, standard read-out equipment is bulky, complex and

Last updated on: 16-03-2022 - 14:23

Contact: Wendy Meulebroeck
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
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Organoid brightfield identification-based therapy screening

We developed a high-throughput live-cell imaging-based organoid analysis platform, Organoid Brightfield Identification-based Therapy Screening (Orbits), that allows for kinetic monitoring of organoid growth and drug responses in routine extracellular matrix domes, high-throughput 384-well

Last updated on: 16-03-2022 - 13:59

Contact: Christophe Deben
Organisation: University of Antwerp (UAntwerpen)
Status: Internally validated
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Prediction of toxicological endpoints by QSAR modeling

Quantitative Structure Activity Relationship modeling is generally used to construct models in which molecular descriptors of chemical compounds are used to predict endpoints/activities of interest. Commercial packages are available that can be implemented, but new models can be constructed if

Last updated on: 16-03-2022 - 13:49

Contact: Geert Verheyen
Organisation: Thomas More University of Applied Sciences
Status: Still in development, History of use, Published in peer reviewed journal
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Culturing HeLa cells

HeLa cells are the first continuous cancer cell line and were isolated from the aggressive glandular cervical cancer of a 31-year old woman. It was the first aneuploid line derived from human tissue maintained in continuous cell culture. Knowledge of almost every process that occurs in human cells

Last updated on: 28-02-2022 - 11:01

Contact: Jessie Neuckermans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development, History of use
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Flow-cytometric determination of neutral lipids

Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417

Last updated on: 28-02-2022 - 11:00

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated
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