Alkaline comet assay (COM) and Micronucleus test (MN) are well-established cytogenetic tests that are used to detect both immediate damages i.e. DNA fragmentation, and permanent damage which results in micronuclei formation. These techniques are often applied separately or, sometimes, in combination

Experiments were conducted using human induced pluripotent stem cells supplemented with hiPSC derived astrocytes and their reagents can be obtained commercially and cultured as per manufacturer’s instructions. One day before plating the cells, each 48-well MEA plate was pre-coated with a

Experiments were conducted using cortical cells that contains glutamatergic and gabaergic neurons and glia. Primary neurons were freshly dissociated from embryonic E18-19 rat cortices as described previously and plated onto 48-well MEA plates (Maestro system, Axion Biosystems). HOne day before