Biobanking of processed mice brains
Scope of the method
- Animal health
- Human health
- Basic Research
- Translational - Applied Research
- In vitro - Ex vivo
- Animal derived cells / tissues / organs
Description
- biobanking
- frozen tissue
- paraffin blocks
- brain slices
- mouse
- IHC and IF stainings
- neuropathology
- tissue biobanking
- biochemistry
- genetics and transcriptomics
Brain from treated and not treated mice is collected after death. Tissue is fixed in 4% PFA for 4 days. After specific cutting (ex. L and R hemisphere) tissue is placed in a cassette. Cassette is placed in Tissue Processor (where water from the tissue is removed and replaced with paraffin). Brain is embedded in paraffin. After paraffin block is ready it can be cut with microtome and slides can be stained or stored. In this method tissue from different mouse strains, different age, treated or not treated mice can be processed and stored for future applications/projects. Slides can be used for different kinds of stainings even years after collecting of the tissue.
- PFA 4% ;
- Perfusion equipment ;
- Paraffin ;
- Tissue Processor ;
- Tissue embedding equipment ;
- Microtome.
- History of use
- Internally validated
Pros, cons & Future potential
Collected, processed and sliced tissue from one mouse can be used for many different projects.
- Big challenge for this method is collecting enough tissue from enough number of mice at different age.
- Space for collected tissue (blocks and slides).
Biobanking can be used for any type of tissue and any kind of lab animal. Another alternative method to replace studies on murine disease models for pathological features is the use of human autopsy tissue that allows to study a disease as well, which is collected in ethically approved recruitment projects. Alternatively, frozen tissue/lysates not entirely needed for a distinct study can also be kept frozen in the biobank for further experiments.
Centralized Animal Biobank may potentially decrease number of animal used in experiments.
References, associated documents and other information
Organisations
Katholieke Universiteit Leuven (KUL)Department of Imaging & Pathology
Belgium
Flemish Region
Katholieke Universiteit Leuven (KUL)
Neuropathology Lab
Belgium
Flemish Region