C. elegans genetics
Scope of the method
- Animal health
- Human health
- Basic Research
- In vivo
- Animal derived cells / tissues / organs
- C. elegans
- genetic screen
- pharmacological screen
- calcium imaging
The lab routinely use C. elegans culture as genetic model. We explore the development and the ageing of the neuromuscular system. The methods used regularly in our lab include molecular biology, generation of transgenic animals or mutants, crossings, genetic screen, calcium imaging, fluorescence imaging, comprehensive quantification of the worm locomotion in response to specific cues, survival curves, molecular biology.
- Internally validated
- Published in peer reviewed journal
Pros, cons & Future potential
- Many genes for the nervous system development and function are conserved.
- Genetic of ageing is established in C. elegans or Drosophila.
- C. elegans genetics is well established, genetic manipulation is quick and easy, and many mutants are available.
- Culture is simple and lead to isogenic cohorts.
- The brain structure has nothing to do with mamalian systems.
- C. elegans lifespan is <1 month.
- C. elegans is an invertebrate, many organs are not conserved.
References, associated documents and other information
Contact personpatrick laurent
OrganisationsUniversité libre de Bruxelles
ULB Institute for Neuroscience