Isolation Femoral and tibial bones are isolated from 10-12 weeks old C57BL/6J mice. After cutting the bone's ends, the bone marrow is flushed out using a 23G syringe with PBS into a 50ml falcon tube. After centrifugation cells are re-suspended in FCS (0.5ml/mouse leg). Differentiation 0.5ml of the

Last updated on: 14-08-2020 - 15:26

Contact: Jerome Hendriks
Organisation: Hasselt University
Status: History of use, Internally validated, Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs

Last updated on: 14-08-2020 - 15:22

Contact: Joy Irobi
Organisation: Hasselt University
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
The feed undergoes an in vitro enzymatic hydrolysis simulating the digestion in the upper digestive tract followed by a dialysis mimicking the intestinal absorption. Finally, an in vitro batch fermentation is performed and simulates the fermentation occurring in the hindgut. The ingredients, ground

Last updated on: 16-07-2020 - 15:04

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
Prebiotics are used by the gut microbiota creating metabolites that exert a positive effect on the host (postbiotics). Prebiotics are available on the market for human and animal consumption and may induce differential effects on the host's health and metabolism. They can be tested by in vitro

Last updated on: 16-07-2020 - 15:02

Contact: Nadia Everaert
Organisation: Liège Université
Status: Published in peer reviewed journal
Cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) offer an attractive platform for cardiovascular research, including disease modeling, drug toxicity testing and development of regenerative therapies. Patient-specific iPSC-CMs are very useful to study disease pathogenesis and

Last updated on: 30-06-2020 - 14:13

Contact: Maaike Alaerts
Organisation: University of Antwerp
Status: Still in development, Internally validated
Vascular smooth muscle cell (VSMC) deficiency plays a pivotal role in aneurysm development. Unfortunately, access to native VSMCs of patients and (particularly) control individuals is extremely limited. It has been shown that iPSC-derived VSMCs recapitulate the yet known disease processes very well.

Last updated on: 03-06-2020 - 13:51

Organisation: University of Antwerp
Status: Still in development, Internally validated
This method is a straightforward, colorimetric, and inexpensive high-throughput screening system in bacteria which depends on the activity of human HPD. This screening assay is based on the formation and accumulation of a melanin-like ochronotic pigment which has a characteristic brown color. In the

Last updated on: 26-05-2020 - 11:30

Organisation: Vrije Universiteit Brussel, RWTH Aachen
Partners: RWTH Aachen
Status: Published in peer reviewed journal
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,

Last updated on: 19-05-2020 - 11:02

Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
A calculated number of bacteria is injected through the pro-legs of the larvae. As several entry points are present, this allows to inject virulent bacteria and potential antimicrobial compounds within the same host. The survival of the larvae is scored over time (days post inoculation) until about

Last updated on: 19-05-2020 - 11:01

Organisation: Vrije Universiteit Brussel
Status: Internally validated, Published in peer reviewed journal
Reverse pharmacology is a high-throughput in vitro method to characterise ligand-receptor interactions. In this method, a receptor of interest is expressed in a heterologous cell line and used as a hook to fish out its ligand(s) from a library of synthetic compounds. Receptor activation is measured

Last updated on: 17-04-2020 - 09:56

Contact: Isabel Beets
Organisation: KU Leuven
Status: Published in peer reviewed journal