VITO can offer expertise in animal-free methods for inhalation testing. VITO has an Air-Liquid Interface (ALI) platform with three ALI exposure modules: two commercial systems for bronchial studies and one in-house developed system for lower airway studies. At VITO ALI exposure modules, aerosol

Last updated on: 08-11-2019 - 14:08

Organisation: VITO
Partners: VITO
Status: History of use, Internally validated, Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which

Last updated on: 08-11-2019 - 14:06

Contact: Yorick Janssens
Organisation: Ghent University
Status: Published in peer reviewed journal
Transport kinetics accross the skin and/or mucosa is investigated using Franz diffusion cells and LC-UV/MS detection of the investigated molecule in the receptor compartment.

Last updated on: 08-11-2019 - 14:05

Contact: Yorick Janssens
Organisation: Ghent University
Status: Internally validated, Published in peer reviewed journal
This work will be based on data describing the rat hindlimb kinematics obtained by X-Ray Reconstruction of Moving Morphology (XROMM) and ground reaction forces following destabilization of the medial meniscus (DMM) and running. These data will be analyzed with multi-body dynamics to estimate the

Last updated on: 08-11-2019 - 14:02

Contact: Judith Piet
Organisation: KU Leuven
Status: Still in development
A compound of interest (e.g. new psychoactive substance, endocrine disrupting compound, ...) is incubated with human liver microsomes and liver cytosolic fractions to generate both Phase I and II metabolites. Samples are prepared for analysis using a simple method in order to avoid possible losses

Last updated on: 08-11-2019 - 14:01

Organisation: University of Antwerp
Status: Published in peer reviewed journal
Follicle Biology Laboratory has developed a well characterized and standardized MOUSE in vitro follicle culture (IFC) system. In this system, early stage ovarian follicles are cultured in vitro under physiological hormone concentrations up to fertilizable and developmentally competent mature oocytes

Last updated on: 08-11-2019 - 14:00

Contact: Ellen Anckaert
Organisation: Vrije Universiteit Brussel
Status: History of use, Internally validated, Published in peer reviewed journal
Three-dimensional (3D) lung epithelial cell models mimic key aspects of the parental tissue, including apical-basolateral polarity and barrier function (Barrila et al. 2010, PMID: 20948552). These 3D cultures are generated using the rotating wall vessel (RWV) bioreactor system, allowing host cells

Last updated on: 08-11-2019 - 13:59

Contact: Aurélie Crabbé
Organisation: Ghent University
Status: Published in peer reviewed journal
The Galleria mellonella assay makes use of larvae of the Greater Wax Moth. G. mellonella larvae are injected with a small amount of microorganisms, and survival can easily be followed for up to 1 week. This allows to compare virulence between different organisms. Alternatively, after injection of

Last updated on: 08-11-2019 - 13:58

Contact: Tom Coenye
Organisation: Ghent University
Status: Published in peer reviewed journal
The C. elegans assay can be used to study the virulence of various microorganisms, and can be used to evaluate the antimicrobial effect of drugs or drug candidates. The two main outcome parameters are (i) survival of C. elegans and (ii) microbial load.

Last updated on: 08-11-2019 - 13:57

Contact: Tom Coenye
Organisation: Ghent University
Status: Published in peer reviewed journal