We have implemented the use of 3D patient-derived tumor organoids, to study metabolic (dys)functions associated with tumor development and progression, including response to anticancer therapies. These sophisticated models allow to better integrate the influence of microenvironmental conditions in

Last updated on: 23-02-2024 - 16:20

Contact: Cyril Corbet
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
Adult intestinal stem cells are grown into closed 3D structures with protruding buds and a luminal compartment. These 3D structures are produced from the stem cells present in the base of crypts isolated from rectal mucosa samples that can be collected by suction or forceps biopsy. The crypts are

Last updated on: 22-02-2024 - 11:15

Contact: Anabela Ramalho
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The application of liver organoids is very promising in the field of liver tissue engineering; however, it is still facing some limitations. One of the current major limitations is the matrix in which they are cultured. The mainly undefined and murine-originated tumor matrices derived from

Last updated on: 22-01-2024 - 17:28

Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Method to culture human epidermal keratinocytes and seed them for tissue reconstruction at the air-liquid interface over a polycarbonate porous membrane.

Last updated on: 19-12-2023 - 19:19

Contact: Yves Poumay
Organisation: Université de Namur (UNamur)
Status: Published in peer reviewed journal
More than 3 million deaths worldwide are linked to excessive alcohol consumption. Alcohol abuse associated gut barrier dysfunction is thought to play an important role in the development of alcohol-associated liver disease. Although some aspects that contribute to this process have been elucidated,

Last updated on: 30-10-2023 - 10:05

Organisation: Université Catholique de Louvain (UCL)
Partners: UCLouvain, Cliniques universitaires Saint-Luc, UCLouvain
Status: Still in development
The maturation of the human brain shows species-specific differences of neoteny when compared to lower mammals. This process encompass a time window that expands from late embryonic stages to early adolescence. Major features of brain maturation are the acquisition of phenotypic complex traits such

Last updated on: 25-09-2023 - 14:38

Organisation: University of Liège (ULiège)
Status: Still in development, History of use
The method is an approach to systematically study the impact of the nutrient microenvironment on the metabolism of effector CD8+ T cells, based on performing stable 13C isotope labeling measurements on in vitro-differentiated murine effector CD8+ T cells. Naive CD8+ T cells are isolated from mouse

Last updated on: 31-08-2023 - 17:12

Contact: Dorien Broekaert
Organisation: Katholieke Universiteit Leuven (KUL), VIB - KU Leuven
Status: Published in peer reviewed journal
Metabolomics and 13C tracer analysis are state-of-the-art techniques that allow determining the concentration of metabolites and the activity of metabolic pathways, respectively. Three dimensional (3D) cultures of cancer cells constitute an enriched in vitro environment that can be used to assay

Last updated on: 31-08-2023 - 17:12

Contact: Dorien Broekaert
Organisation: VIB - KU Leuven, Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
This assay evaluates the sprouting ability of endothelial cells in a collagen matrix and it used as an in vitro model to study the formation of new blood/lymphatic vessels. The effect of pro-angiogenic growth factors or co-cultured cells can be measured by quantifying the amount of vascular sprouts

Last updated on: 25-08-2023 - 09:11

Contact: Diede Houbaert
Organisation: Cellular and Molecular Medicine - KU Leuven
Status: History of use, Internally validated, Published in peer reviewed journal