Primary epithelial stem/progenitor cell populations are isolated from the murine lung using fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS). Purified epithelial stem/progenitor cells are grown together with mesenchymal cells in Matrigel, a material enriched for

Last updated on: 15-02-2023 - 10:50

Contact: Smole Ursula
Organisation: VIB - UGent
Status: Still in development, Published in peer reviewed journal
We devised a simple and reliable method for reprogramming peripheral blood mononuclear cells into hiPSC and then to differentiate them into air-liquid interface bronchial epithelium (iALI) within 45 days. Of note, this method does not involve any cell sorting step. We reprogrammed blood cells from

Last updated on: 07-02-2023 - 14:46

Contact: Engi Ahmed
Organisation: VIB - UGent, IRMB INSERM 1183
Status: History of use, Internally validated, Published in peer reviewed journal