Generally, our research group aims to study the influence of food parameters (food design, food processing, food composition) on the digestive kinetics of diverse nutrients throughout the upper gastrointestinal tract. For this, we use in vitro digestion protocols. The current method is a semi

Last updated on: 15-02-2023 - 16:08

Contact: Tara Grauwet
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Primary epithelial stem/progenitor cell populations are isolated from the murine lung using fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS). Purified epithelial stem/progenitor cells are grown together with mesenchymal cells in Matrigel, a material enriched for

Last updated on: 15-02-2023 - 10:50

Contact: Smole Ursula
Organisation: VIB - UGent
Status: Still in development, Published in peer reviewed journal
This method is used to let cells interact for a better simulation of processes occurring in the body. The rational of the method is that monoculture experiments do not capture the complexity of in vivo interactions between different organs. In the most simple setup (published work Nutrients), Caco-2

Last updated on: 09-02-2023 - 16:37

Contact: Amar van Laar
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
We devised a simple and reliable method for reprogramming peripheral blood mononuclear cells into hiPSC and then to differentiate them into air-liquid interface bronchial epithelium (iALI) within 45 days. Of note, this method does not involve any cell sorting step. We reprogrammed blood cells from

Last updated on: 07-02-2023 - 14:46

Contact: Engi Ahmed
Organisation: VIB - UGent, IRMB INSERM 1183
Status: History of use, Internally validated, Published in peer reviewed journal
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. Considering the difficulty to work on dendritic

Last updated on: 03-02-2023 - 08:51

Contact: Fiona Ingrao
Organisation: Sciensano
Status: Still in development
Ex-vivo tissue explants (precision cut tissue slices) prepared with the Krumdieck Tissue Slicer are living, three-dimensional tissue slices closely resemble the organ from which it is prepared, with all the cell types present in their original tissue-matrix configuration where physiological and

Last updated on: 31-01-2023 - 15:52

Contact: Bella Manshian
Organisation: Katholieke Universiteit Leuven (KUL)
Human Intestinal Organoids (HIOs) are in vitro 3D cell cultures arranged in a crypt-villus structure that incorporate many physiological features of the intestinal epithelium, including the presence of different cell populations (enterocytes, goblet cells, enteroendocrine and Paneth cells). HIOs can

Last updated on: 27-01-2023 - 13:29

Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Overuse tendon injuries are a major cause of musculoskeletal morbidity in both human and equine athletes. Despite the big burden, there is no effective treatment to restore tendon’s natural composition, due to a lack of understanding of fundamental cell biology. Additionally, translation of novel

Last updated on: 17-01-2023 - 16:22

Organisation: Ghent University (UGent)
Status: Still in development
Intestinal organoids are cultured from intestinal biopsies obtained during routine endoscopy. The stem cell containing crypts are isolated and cultured in a 3D ECM (Matrigel) in the presence of the desired growth factors. The present stem cells will expand and give rise to all epithelial cells of

Last updated on: 11-01-2023 - 16:43

Contact: Bram Verstockt
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
Myeloid progenitor cells derived from the bone marrow of mice are stimulated using a cytokine mixture to become myeloid derived suppressor cell (MDSC) like cells. These are co-cultured with activated CD8+ T-cells derived from a mouse spleen. The MDSC like cells will suppress the proliferation of the

Last updated on: 10-01-2023 - 16:48

Contact: Yani Berckmans
Organisation: Katholieke Universiteit Leuven (KUL), Oncology - KU Leuven
Status: History of use, Published in peer reviewed journal