This is a method to study host-microbe interaction in the upper respiratory tract. A physiologically representative epithelial structure, with mucin producing and ciliated cells, is obtained by culturing respiratory epithelial cells at air-liquid interface in Transwell inserts. Optionally,

Last updated on: 25-02-2020 - 23:58

Organisation: Ghent University
Partners: Biotech/TU-Dresden
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal

We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the

Last updated on: 04-02-2020 - 13:31

Organisation: Ghent University, Ghent University, Liège Université
Status: Internally validated

The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured

Last updated on: 26-11-2019 - 13:12

Contact: Kristel Demeyere
Organisation: Ghent University
Status: Published in peer reviewed journal

Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e

Last updated on: 12-11-2019 - 11:34

Contact: Maarten Dhaenens
Organisation: Ghent University
Status: Still in development, Internally validated, Published in peer reviewed journal

It is the goal of the DNA-adductomics to search for DNA-adducts that might be formed during interaction with contaminants. The analysis of DNA adducts is performed using ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the

Last updated on: 12-11-2019 - 11:16

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: Internally validated, Published in peer reviewed journal

Our polar metabolomics profiling and fingerprinting methodology applies ultra-high performance liquid chromatography coupled to hybrid quadrupole-Orbitrap high resolution mass spectrometry. Both the instrumental method, as well as generic extraction protocols for colon tissue, cell cultures,

Last updated on: 12-11-2019 - 11:13

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: Internally validated, Published in peer reviewed journal

The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be

Last updated on: 12-11-2019 - 10:56

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: Published in peer reviewed journal

Our lipidomics profiling and fingerprinting methodology enables the analysis of all lipids within a biological matrix. These lipids can then be compared between an individual or condition with a specific metabolic state and healthy controls to find biomarkers or pathways that can be related to

Last updated on: 08-11-2019 - 14:12

Contact: Lynn Vanhaecke
Organisation: Ghent University
Status: History of use, Internally validated, Published in peer reviewed journal