This method describes a well-known optimised human in vitro model of drug-induced cholestasis. Cryopreserved primary human hepatocytes are cultured between two layers of extracellular matrix scaffold, which will delay dedifferentiation and allows to restore cell-extracellular matrix interactions.

Last updated on: 28-02-2022 - 11:00

Contact: Eva Gijbels
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Using this method you can measure the (relative) lipid load in human skin-derived stem cells differentiated towards hepatic cells. This method could also be applied on other cell types (e.g. HepG2), since it is based on the following publication: "M. T. Donato et al., Chem. Biol. Interact. 181, 417

Last updated on: 28-02-2022 - 11:00

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated
Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells exhibit a

Last updated on: 28-02-2022 - 11:00

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation occurs

Last updated on: 28-02-2022 - 11:00

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a

Last updated on: 28-02-2022 - 11:00

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Polycaprolactone (PCL) fiber materials are fabricated using an electrospinning method. Molecular weight of PCL is 45 000 Da (Sigma-Aldrich). The electrospinning process is performed using 18wt% PCL solution dissolved in chloroform:ethanol at a ratio of 9:1. Two high-voltage sources are used to

Last updated on: 28-02-2022 - 11:00

Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the Burker

Last updated on: 28-02-2022 - 10:59

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
ATP transports chemical energy within cells by serving as a substrate for kinases and as such fulfills a vital function in numerous cellular processes such as cell injury and subsequent cell death. ATP is therefore a crucial player in these events that are results of intracellular stress.

Last updated on: 28-02-2022 - 10:59

Contact: Axelle Cooreman
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
The MTT test is performed to determine the in vitro cytotoxicity of selected chemicals. The mitochondrial enzyme succinate deydrogenase is responsible for the biotransformation of toxic agents and MTT. The ability of cells to reduce MTT provides an indication of the mitochondrial integrity and

Last updated on: 28-02-2022 - 10:59

Contact: Axelle Cooreman
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
The standard operating procedure describes a method to assess the cholestasis-inducing potential of chemicals, in casu in cultures of primary rat hepatocytes. The procedure relies on the accumulation of the fluorescent bile salt export pump (Bsep) substrate cholyl-lysyl-fluorescein (CLF) in the

Last updated on: 28-02-2022 - 10:59

Organisation: Vrije Universiteit Brussel (VUB)