The method uses planarians as an alternative in vivo model to assess carcinogenicity. Planarians are characterized by a high regenerative capacity and a large number of pluripotent stem cells. The assay is based on the discriminative power of stem cells in an in vivo setting. Based on specific stem
Last updated on: 28-03-2024 - 08:56
Contact: Karen Smeets
Organisation: University of Hasselt (UHasselt)
Status: Internally validated, Published in peer reviewed journal
Endotoxins are lipopolysaccharides anchored at the outer membrane of gram-negative bacteria. These pyrogenic compounds can be introduced in pharmaceutical products during their manufacture and could induce severe physiological reactions in humans. This is why endotoxins are dosed, in order to assure
Last updated on: 28-09-2023 - 11:59
Contact: Flore Laurent
Organisation: Sciensano
Status: Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Severely burned skin can exhibit serious contractions that may negatively impact the mobility of joints of patients. The method deals with post-burn evolution of skin, in which one considers the balance of momentum, cells, collagen and chemokines. The balances are represented in terms of partial
Last updated on: 05-09-2023 - 11:43
Contact: Fred Vermolen
Organisation: University of Hasselt (UHasselt)
Status: Still in development, Internally validated, Published in peer reviewed journal
NMTox is an R-software package and a Shiny app that can be used to explore and subset large datasets and can identify and test for monotonic dose responses. The package was developed within the NanoInformaTIX project where a platform is developed that aims to predict nanomaterial toxicity.
Last updated on: 22-08-2023 - 10:43
Contact: Geert Verheyen
Organisation: Thomas More University of Applied Sciences, University of Hasselt (UHasselt)
Status: Internally validated
Using an in vitro flow system adhesion assay, immune cell adhesion to (blood-brain barrier) endothelial cells can be quantified under physiological blood flow conditions. Endothelial cells can be treated with inflammatory cytokines or therapeutic antibodies to mimic inflammatory diseases. Immune
Last updated on: 06-06-2023 - 10:35
Contact: Doryssa Hermans
Organisation: University of Hasselt (UHasselt)
Status: Published in peer reviewed journal
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. Considering the difficulty to work on dendritic
Last updated on: 03-02-2023 - 08:51
For the risk assessment of compounds migrating from food contact materials (FCM), information on the exposure to the migrant as well as its possible hazards is needed. To support the evaluation of both starting products and NIAS from plastic FCM, the VERMEER FCM tool has been developed within the
Last updated on: 29-11-2022 - 14:46
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that
Last updated on: 23-11-2022 - 16:31
Contact: Birgit Mertens
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
The VITOTOX test is a bacterial genotoxicity test. The test is based on bacteria that contain the lux operon of Vibrio fischeri under transcriptional control of the mutated recN promoter, which is controlled by the bacterial SOS-system (TA 104-recN2-4 strain or Genox strain). After incubation
Last updated on: 26-05-2022 - 10:40
Contact: Roel Anthonissen
Organisation: Sciensano
Status: History of use, Published in peer reviewed journal
The CALUX® system (Chemically Actived LUciferase eXpression) of Bio Detection Systems (BDS, Amsterdam, the Netherlands) uses U-2 OS cells (human osteoblast) that are stably transfected with human TRb or human PPARg2 (BDS, Amsterdam) and a luciferase reporter construct under the control of a receptor
Last updated on: 05-05-2022 - 08:59
Contact: Cannot be disclosed
Organisation: Sciensano
Partners: BDS
Status: Published in peer reviewed journal
