We have implemented the use of 3D patient-derived tumor organoids, to study metabolic (dys)functions associated with tumor development and progression, including response to anticancer therapies. These sophisticated models allow to better integrate the influence of microenvironmental conditions in

Last updated on: 23-02-2024 - 16:20

Contact: Cyril Corbet
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
The chick chorioallantoic membrane (CAM) model is particularly attractive to study short-term xenografting of human ovarian tissue. Its angiogenic potential and natural immunodeficiency allow scrutiny of early follicle activation and loss and graft revascularization mechanisms. Chick embryo

Last updated on: 14-02-2024 - 17:24

Organisation: Université Catholique de Louvain (UCL)
Status: Internally validated, Published in peer reviewed journal
More than 3 million deaths worldwide are linked to excessive alcohol consumption. Alcohol abuse associated gut barrier dysfunction is thought to play an important role in the development of alcohol-associated liver disease. Although some aspects that contribute to this process have been elucidated,

Last updated on: 30-10-2023 - 10:05

Organisation: Université Catholique de Louvain (UCL)
Partners: UCLouvain, Cliniques universitaires Saint-Luc, UCLouvain
Status: Still in development
Endotoxins are lipopolysaccharides anchored at the outer membrane of gram-negative bacteria. These pyrogenic compounds can be introduced in pharmaceutical products during their manufacture and could induce severe physiological reactions in humans. This is why endotoxins are dosed, in order to assure

Last updated on: 28-09-2023 - 11:59

Contact: Flore Laurent
Organisation: Sciensano
Status: Internally validated, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Macrophages orchestrate reactive particle segregation, compact aggregates of immune cells and non-immune cells and promote fibrosis-surrounding granulomas. We developed a simple 3D in-vitro model that mimics granuloma formation and categorizes granuloma-inducing inorganic particles. Macrophage

Last updated on: 03-04-2023 - 11:21

Contact: Léa Hiéronimus
Organisation: Université Catholique de Louvain (UCL)
Status: Still in development, Internally validated
We aim to differentiate ovarian cells isolated from postmenopausal ovaries into steroidogenic theca interna cells. For this purpose, cryopreserved ovarian cortex fragments are used to isolate ovarian cells. These cells are cultured on collagen and supplemented with growth factors defined to promote

Last updated on: 20-03-2023 - 13:52

Contact: Hanne Vlieghe
Organisation: Université Catholique de Louvain (UCL)
Status: Published in peer reviewed journal
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. Considering the difficulty to work on dendritic

Last updated on: 03-02-2023 - 08:51

Contact: Fiona Ingrao
Organisation: Sciensano
Status: Still in development
The goal of chicken embryonic spinal cord electroporation is to increase or to reduce expression levels of genes of interest in the developing spinal cord, and to assess the phenotypic consequences of these alterations on neuronal differentiation or migration. Fertilized eggs stored at 14°C are

Last updated on: 13-12-2022 - 11:23

Organisation: Université Catholique de Louvain (UCL)
Status: History of use, Internally validated, Published in peer reviewed journal
For the risk assessment of compounds migrating from food contact materials (FCM), information on the exposure to the migrant as well as its possible hazards is needed. To support the evaluation of both starting products and NIAS from plastic FCM, the VERMEER FCM tool has been developed within the

Last updated on: 29-11-2022 - 14:46

Organisation: Sciensano
Status: Internally validated
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. Micronuclei may originate from acentric chromosome fragments (i.e. lacking a centromere), or whole chromosomes that

Last updated on: 23-11-2022 - 16:31

Contact: Birgit Mertens
Organisation: Sciensano
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)