Isolation Femoral and tibial bones are isolated from 10-12 weeks old C57BL/6J mice. After cutting the bone's ends, the bone marrow is flushed out using a 23G syringe with PBS into a 50ml falcon tube. After centrifugation cells are re-suspended in FCS (0.5ml/mouse leg). Differentiation 0.5ml of the

Last updated on: 14-08-2020 - 15:26

Contact: Jerome Hendriks
Organisation: Hasselt University
Status: History of use, Internally validated, Published in peer reviewed journal
Characterization of Human Oligodendroglia derived-EV-rich in sHSPs (HOG-EV-sHSPs). Establish HSPB1/B8 stably expressing cell lines for the production of sHSPs rich-EV cell culture conditioned medium. Human Oligodendroglia cell lines (HOG) were stably transduced with pLenti-HSPBs gene constructs

Last updated on: 14-08-2020 - 15:22

Contact: Joy Irobi
Organisation: Hasselt University
Status: Still in development, Internally validated, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
Peripheral blood mononuclear cells (PBMC) are isolated from the peripheral blood using Ficoll gradient centrifugation. The PBMC are then stained with fluorescently-labelled monoclonal antibodies directed against cell surface or intracellular proteins that can be used to identify specific immune cell

Last updated on: 03-03-2020 - 11:46

Contact: Cannot be disclosed
Organisation: Hasselt University
Status: Published in peer reviewed journal
Cell line which is commonly used to study biological processes in mouse macrophages. It is a macrophage-like cell line derived from BALB/c mice.

Last updated on: 25-02-2020 - 23:58

Contact: Jerome Hendriks
Organisation: Hasselt University
Status: History of use
Worldwide, outdoor air pollution is responsible for 4.2 million premature deaths per year. Both chronic and acute exposure to particulate matter air pollution is a risk factor for heart and lung diseases. One of the atmospheric pollutant particles is represented by soot or black carbon (BC)

Last updated on: 15-11-2019 - 16:16

Contact: Eva Bongaerts
Organisation: Hasselt University
Partners: Hasselt University, KU Leuven
Status: History of use, Internally validated, Published in peer reviewed journal
TEM, NTA and western blot were used to characterize the purified inflammation‐associated EV subpopulations from TNF-α treated HUVEC based on their sedimentation speeds (10K and110K) and surface markers (CDs and ICAM-1). Protein arrays were used to discover the immunomodulatory content of

Last updated on: 14-11-2019 - 14:02

Organisation: Hasselt University
Status: Published in peer reviewed journal, Currently submitted for further validation by an external party (e.g. OECD, EURL ECVAM,…)
This method describes the steps from a living mouse to a multi-cellular brain slice model where complex cellular interactions can be evaluated.

Last updated on: 08-11-2019 - 13:14

Contact: Melissa Schepers
Organisation: Hasselt University
Status: Still in development