The response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol was investigated using primary sublingual epithelial cells isolated from dogs and cultured in vitro. After 24-h incubation with

Last updated on: 07-04-2021 - 14:52

Contact: Eric Cox
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Retinal gene delivery via intravitreal injection is hampered by various physiological barriers present in the eye of which the vitreoretinal (VR) interface represents the most serious hurdle. We present a retinal explant model especially designed to study the role of this interface as a barrier for

Last updated on: 24-03-2020 - 16:26

Contact: Katrien Remaut
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Mononuclear cells are flushed from the bone marrow using a 25G needle. After centrifugation, the cells are resuspended in 50mL pre-heated DMEM containing 20ng/ml M-CSF. The cells are cultured in 10cm petri dishes for 7 days. At days 2,4 and 6, the medium is renewed after washing away non-adherent

Last updated on: 10-03-2020 - 10:17

Contact: Cannot be disclosed
Organisation: Ghent University (UGent)
Partners: Ghent University hospital (UZ Gent)
Status: Published in peer reviewed journal
This is a method to study host-microbe interaction in the upper respiratory tract. A physiologically representative epithelial structure, with mucin producing and ciliated cells, is obtained by culturing respiratory epithelial cells at air-liquid interface in Transwell inserts. Optionally,

Last updated on: 28-02-2020 - 10:51

Organisation: Ghent University (UGent)
Status: Still in development, History of use, Internally validated, Published in peer reviewed journal
We have developed an innovative two-chamber skin explant model specifically for studies involving skin of marine fish. An apparatus was 3D-printed and consists of two plates, a lower and upper plate (with funnel). By mounting the explant between the plates, two chambers can be created with the

Last updated on: 04-02-2020 - 13:31

Organisation: Ghent University (UGent), University of Liège (ULiège)
Status: Internally validated
We describe a fluorescent cell-based in vitro infection model that reproduces host-Batrachochytrium dendrobatidis (Bd) interactions. Using the epithelial cell line A6 from Xenopus laevis, we reproduced different stages of host cell infection and intracellular growth of Bd, resulting in host cell

Last updated on: 10-01-2020 - 15:28

Contact: Elin Verbrugghe
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
The BALB/c-derived - 4T1 mammary tumor cell line and RAW264.7 macrophage cell line were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in culture flasks. Harvesting of cultured

Last updated on: 26-11-2019 - 13:12

Contact: Kristel Demeyere
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Epigenetics have taken centre stage in the study of diseases such as cancer, diabetes, and neurodegeneration. A new field is emerging that targets epigenetic modifications for therapeutic intervention: pharmacoepigenetics. Particularly in oncology, inhibitors of epigenetic-modifying proteins, i.e.

Last updated on: 12-11-2019 - 11:34

Contact: Maarten Dhaenens
Organisation: Ghent University (UGent)
Status: Still in development, Internally validated, Published in peer reviewed journal
The aim of these in vitro digestions is to simulate the gastrointestinal digestion of specific food sources and to identify metabolites that might be formed out of this food source by the residing microbiome. For this purpose, fecal samples will be collected from volunteers and will be prepared as

Last updated on: 12-11-2019 - 10:56

Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Cells are treated with compounds and after a specific incubation time the MTT reagent is added. In living cells, the MTT is reduced to the purple formazan. The amount of formazan is related to the amount of living cells. Next, the medium and MTT reagent are aspirated and DMSO is added after which

Last updated on: 08-11-2019 - 14:06

Contact: Yorick Janssens
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal