The in vitro rumen fermentation technique is used to simulate the rumen function/fermentation. The rumen is the first compartment of the digestive tract of ruminants, in which feed - consumed by ruminants - is fermented by rumen microbes. For optimal fermentation, the rumen provides an anaerobic

Last updated on: 07-09-2022 - 16:36

Contact: Veerle Fievez
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
An artificial dog with real skeleton to excercise positioning for radiographic examination and palpation.

Last updated on: 25-07-2022 - 09:53

Organisation: Ghent University (UGent)
Status: Internally validated
This method provides a rapid untargeted screening metabolomics method, which can be used to evaluate whether or not metabolic changes can be appointed according to the metabolic state in your matrix of choice. It applies an ambient ionization technique in which a laser beam or diathermy probe is

Last updated on: 31-03-2022 - 15:31

Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
OECD TG 249 was developed by Prof. Dr. Kristin Schirmer, from Eawag. The OECD 249 aims at assessing the acute toxicity of single or mixtures of chemicals using the permanent rainbow trout gill cell line, RTgill-W1. RTgill-W1 cells in a confluent monolayer in 24-well plates are exposed to the test

Last updated on: 30-03-2022 - 17:15

Contact: João Barbosa
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal, Validated by an external party (e.g. OECD, EURL ECVAM,…)
Maaike Vercauteren developed the innovative in vitro 'two-chamber skin explant model'. Pieces of skin are kept and examined in a controlled laboratory environment. This is no sinecure, because the skin must continue to function as if it were still attached to the fish. However, the tested setup

Last updated on: 22-02-2022 - 15:13

Contact: Cannot be disclosed
Organisation: Instituut voor Landbouw-, Visserij- en Voedingsonderzoek (ILVO), Ghent University (UGent)
Status: Internally validated
A set of protocols to combine the widely used Caco-2 cell line with digests from in vitro digestion models (small intestine, colon) to study toxicity, intestinal barrier integrity, bioavailability and, when combined with other cell models (immune, liver, endothelium), bioactivity of food related

Last updated on: 18-02-2022 - 08:42

Organisation: Ghent University (UGent)
Status: History of use, Published in peer reviewed journal
Using respirometry (oxygen and pH, XF96 Analyzer), computer assisted cell analysis, and specific substrates and stressors, mitochondrial function and metabolic changes in a diverse set of cell lines can be measured. Relevant to study substrate preferences, acute and chronic effects of toxic

Last updated on: 15-02-2022 - 15:31

Organisation: Ghent University (UGent)
Status: History of use, Published in peer reviewed journal
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase

Last updated on: 11-02-2022 - 10:29

Contact: Olivier De Wever
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal
Advances in cell culture methods allowed 3D culture of gut epithelial cells derived from adult intestinal epithelial stem cells in mice and humans. We show that starting from crypts cultured in an extracellular matrix hydrogel together with essential growth factors, gut organoids can be grown from

Last updated on: 20-01-2022 - 16:14

Contact: Bert Devriendt
Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal
Advances in three-dimensional (3D) culture techniques have shown several advantages over 2D cultures, especially by more accurately mimicking the in vivo environment. This has led to improved reproducibility and reliability of experimental results, which are important criteria in disease modelling

Last updated on: 14-10-2021 - 16:59

Organisation: Ghent University (UGent)
Status: Still in development, Published in peer reviewed journal