Human skin-derived adult stem cells differentiated towards hepatic cells (hSKP-HPC) are used in this method (R. M. Rodrigues et al., Stem Cells Dev. 23, 44–55 (2014)). These cells are exposed to a cocktail of insulin and glucose at certain concentrations. After 24h of exposure, these cells exhibit a

Last updated on: 28-02-2022 - 11:00

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
Non-alcoholic fatty liver disease (NAFLD) ranges from simple steatosis to severe, life-threatening non-alcoholic steatohepatitis (NASH). Steatosis is mostly asymptomatic and does not cause health complications. However, in 5-10% of the cases it proceeds to NASH in which hepatic inflammation occurs

Last updated on: 28-02-2022 - 11:00

Contact: Joost Boeckmans
Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
The effect of fluidics that mimic the blood flow in the liver sinusoids, is evaluated during the hepatic differentiation of human skin-derived precursors (hSKP). In a standard bi-dimensional (2D) cell culture system, hSKP are differentiated to hSKP-HPC for 24 days in static conditions. In a

Last updated on: 28-02-2022 - 11:00

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
For the generation of the human skin-derived precursors (hSKP) spheroids, ultra-low attachment 96-well plates, cell-repellent (Greiner) are used. hSKP cultured on tissue culture plates are detached with trypple (5 minutes) and collected in a tube. After centrifugation, hSKP are counted at the Burker

Last updated on: 28-02-2022 - 10:59

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
The standard operating procedure describes a method to assess the cholestasis-inducing potential of chemicals, in casu in cultures of primary rat hepatocytes. The procedure relies on the accumulation of the fluorescent bile salt export pump (Bsep) substrate cholyl-lysyl-fluorescein (CLF) in the

Last updated on: 28-02-2022 - 10:59

Organisation: Vrije Universiteit Brussel (VUB)
The standard operating procedure for Sudan Red III in situ staining of cultured rat hepatocytes describes how to detect one of the aspects of drug-induced cytotoxicity i.e. the intracellular accumulation of lipids or in other words steatosis, in primary rat hepatocyte cultures. It is based on the

Last updated on: 28-02-2022 - 10:59

Organisation: Vrije Universiteit Brussel (VUB)
This method describes a very reliable and robust in vitro model for the screening of the cholestatic liability of drugs and other chemical entities. The 3D spheroids generated from primary human hepatocytes can be cultivated up to 28 days, allowing long-term exposures which can depict otherwise

Last updated on: 28-02-2022 - 10:59

Organisation: Vrije Universiteit Brussel (VUB)
Status: Still in development
The method detects two facets of drug-induced cytotoxicity i.e. the intracellular accumulation of phospholipids and of neutral lipids, i.e. phospholipidosis and steatosis respectively. The assay makes use of a kit containing an aqueous, red-fluorescent formulation of labelled phospholipids (LipidTOX

Last updated on: 28-02-2022 - 10:58

Contact: Kaat Leroy
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Human embryonic kidney (HEK) 293 FT cells is a celline that is very easy to culture and is used to obtain high viral titers. “293” is a reference to the 293th experiment wherein the cell line was discovered. A transfection with an adenovirus type 5 DNA fragment took place, causing the cell line to

Last updated on: 28-02-2022 - 10:58

Contact: Matthias Rombaut
Organisation: Vrije Universiteit Brussel (VUB)
Status: History of use
Spheroids are three-dimensional cellular models with widespread basic and translational application across academia and industry. However, methodological transparency and guidelines for spheroid research have not yet been established. The MISpheroID Consortium developed a crowdsourcing knowledgebase

Last updated on: 11-02-2022 - 10:29

Contact: Olivier De Wever
Organisation: Ghent University (UGent)
Status: Published in peer reviewed journal