In vitro differentiation of hematopoietic stem cells (HSC) or inducible pluripotent stem cells (IPS) to megakaryocytes and platelets using specific differentiation conditions (liquid and 3D media). CRISPR/cas mutagenesis of HSC or IPS to study the effect of gene depletion or specific mutants on

Last updated on: 26-05-2022 - 10:41

Contact: Kathleen Freson
Organisation: Katholieke Universiteit Leuven (KUL)
Status: Still in development, Internally validated, Published in peer reviewed journal
The purpose of the present development is to use avian MoDCs to implement a cellular platform to increase understanding of the immune responses induced by various antigens of interest (e.g. vaccine candidates) and evaluate their immunogenic potential. Considering the difficulty to work on dendritic

Last updated on: 17-05-2022 - 17:00

Contact: Fiona Ingrao
Organisation: Sciensano
Status: Still in development
This method provides a rapid untargeted screening metabolomics method, which can be used to evaluate whether or not metabolic changes can be appointed according to the metabolic state in your matrix of choice. It applies an ambient ionization technique in which a laser beam or diathermy probe is

Last updated on: 31-03-2022 - 15:31

Contact: Lynn Vanhaecke
Organisation: Ghent University (UGent)
Status: Internally validated, Published in peer reviewed journal
In silico tools are computer-assisted methodologies with a high-throughput that allow to predict the toxic potential of compounds without experimental testing. Consequently, in silico tools are time-, cost- and animal-saving in nature. The most commonly used methods are (quantitative) structure

Last updated on: 24-03-2022 - 11:25

Contact: Birgit Mertens
Organisation: Sciensano
Status: Published in peer reviewed journal
The tetanus neurotoxin (TeNT) is one of the most toxic proteins known to man, which prior to the use of the vaccine against the TeNT producing bacteria Clostridium tetani, resulted in a 20% mortality rate upon infection. The clinical detrimental effects of tetanus have decreased immensely since the

Last updated on: 22-03-2022 - 16:49

Contact: Celine Vanhee
Organisation: Sciensano
Status: Published in peer reviewed journal
Lung cancer thrives in a complex multicellular tumor microenvironment (TME) that impacts tumor growth, metastasis, response, and resistance to therapy. While orthotopic murine lung cancer models can partly recapitulate this complexity, they do not resonate with high-throughput immunotherapeutic drug

Last updated on: 16-03-2022 - 14:53

Contact: Cleo Goyvaerts
Organisation: Vrije Universiteit Brussel (VUB)
Partners: Institut Curie
Status: Published in peer reviewed journal
We established a protocol to efficiently generate hepatic stellate cells (HSCs) from human pluripotent stem cells (PSCs). Our procedure generated complex in vitro spheroid cultures that better mimic the complexity of the liver as well as liver function. In co-culture, iPSC-HSCs promote maintenance

Last updated on: 16-03-2022 - 14:49

Organisation: Vrije Universiteit Brussel (VUB)
Partners: Katholieke Universiteit Leuven (KUL)
Status: Published in peer reviewed journal
The CALUX method is an in vitro bioassay that uses reporter gene cell lines that have been stably transfected with a luciferase reporter gene under the control of relevant receptor specific DNA response element. This enables the screening for chemicals that can bind to specific receptors and

Last updated on: 16-03-2022 - 14:47

Contact: Imke Boonen
Organisation: Vrije Universiteit Brussel (VUB)
Amoebae are natural eukaryotic predators of bacteria, yeasts, fungi and they are ubiquiste. They are excellent and easy-to-use cellular infection models, as they allow to co-cultivate any organisms in a broad range of infection medium, compatible with high quality microscopy techniques,

Last updated on: 16-03-2022 - 14:45

Organisation: Vrije Universiteit Brussel (VUB)
Status: Internally validated, Published in peer reviewed journal